a few emails :)
Artem
On Thu, Jan 9, 2025, 20:50 Marco Bravo wrote:
> Hi Artem!
> Thank you for the suggestions, do you have recommendation of common
> protein-DNA crosslinking methods for crystalization? I suppose I also
> Identified de-stablizing buffers with my thermal shift assa
https://pmc.ncbi.nlm.nih.gov/articles/PMC3901029/
One that comes to mind... not sure if this is what you are looking for.
Artem
On Thu, Jan 9, 2025, 20:23 Chandra Verma <
64fcc5b1f9c3-dmarc-requ...@jiscmail.ac.uk> wrote:
> ARES PRIVATE
>
> Are there examples where anyone h
#3, or perhaps the flying
spaghetti monster; all these options should work equally well.
There are dozens of possible options and it is hard to say what might work
for you specifically without more details.
Artem
On Thu, Jan 9, 2025, 20:01 Marco Bravo <
d0eb7bee83ae-dmarc-requ...@jis
mutations in the
protease, such that the native protease does not cleave any more.
Happy to help further via PM.
Artem
On Thu, Dec 12, 2024, 11:36 Gloria Borgstahl wrote:
> Hello friends, Yet another kinda off topic question from me. I am looking
> into SUMO tags for my protein of inter
want to check is the purity of your reagents,
especially DMPC - I've encountered batch issues with some of the ahem...
*more affordable* suppliers in the past. Sometimes being a cheapskate is a
bad thing.
Artem
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On Mon, Dec 2, 2024 at 11:56 AM Rafael Marques
wr
ek or so).
Last time i bought one, it was reasonably budget friendly (Oryx may be
still more affordable, I am not sure to be honest).
Artem
On Mon, Dec 2, 2024, 08:35 Sarah Bowman <
ad1e26f054b6-dmarc-requ...@jiscmail.ac.uk> wrote:
> We use the Formulator from Formulatrix for mixing
Some have a 'nipple' style valve, others
have a needle style valve and some are permanently welded shut... it's not
obvious that your mechanics shop will have the requisite vacuum connector
to service your shipper. We used to send ours to manufacturer for
reconditioning.
Best of luck,
ystallization, needless to say.
Best of luck in your endeavors!
Artem
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On Wed, Oct 2, 2024 at 2:51 PM amit gaur wrote:
> Hi everyone,
>
> I am trying to crystallize a protein with a drug molecule. The protein
> concentration is 15.5 mg/ml, the
your outcome somehow (e.g. by small
molecule MS).
It's not like this reaction is hard to set up, or requires massive
optimization of conditions - generally speaking, as long as you have 'much
more' of DTT or BME than you have of the covalent agent, given some time
the reaction w
ed silica gel beads in a clean sock should do the trick.
With warmest regards,
Artem
On Fri, Jul 26, 2024 at 11:09 AM Markus Seeliger
wrote:
> Dear All,
> apologies for the off-topic question. I am facing the problem of running
> enzyme activity assays at low temperature (close to 4C woul
displays one of the cutest symmetry
transitions that I've seen so far.
Artem
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On Thu, Jun 27, 2024 at 8:46 AM Andrew Lovering
wrote:
> Dear wise list,
>
>
>
> I have a question regarding protein oligomers that have multiple,
> dif
At this resolution you should be able to infer differences between C and O
based on the map levels at each location. Another dead giveaway is the bond
lengths and the asymmetry of the top region - that is not a carboxylic acid
to me.
This looks lime MPD to me, very classic shape.
Artem
On Wed
the better) and performing harvest
in a cold room
5. flooding the drop with cryo (20-25% ethylene glycol is my #1 choice, but
you need multiple drops to play around with different cryos of course)
Best of luck!
Artem
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On Thu, May 30, 2024 at 7:26 AM 白雪慧 wro
where you
absolutely have to treat domains as separately mobile rigid bodies.
I would love to help more but it would require more sharing that you might
be justifiably unwilling or unable to do :)
Best of luck,
Artem
On Mon, Feb 19, 2024 at 6:51 PM Artem Evdokimov
wrote:
> Hello Ma
, HRDC, etc.)
Hope this helps.
Artem
On Mon, Feb 19, 2024, 5:54 PM Marco Bravo wrote:
> Hello all,
> I recently collected data on some plate crystals for a previously
> uncharacterized protein at the ALS light source. The XDS auto-data
> processing log output indicates that my r
Ebay to the rescue:
https://www.ebay.com/itm/185248167872
is this the correct connector?
Once you have a single connector, you can pin-trace it to your hearts'
delight and make new ones for pennies, since the cable and the plugs are
standard...
Artem
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Could be dna crystals. They often do not diffract.
Artem
On Wed, Nov 8, 2023, 11:18 AM careinaedgo...@yahoo.com <
02531c126adf-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> The reservior solution is 0.2 M NaCl2, 0.1 M HEPES pH 7.5, and 25% 3350 PEG
>
> Protein buffer is 300 mM
n
like lattice. Of course this requires hands on access to a diffractometer,
which these days isn't a common thing.
All the best,
Artem
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On Wed, Nov 8, 2023 at 10:02 AM careinaedgo...@yahoo.com <
02531c126adf-dmarc-requ...@jiscmail.ac.uk>
speaking, resin affinity is only about 1 uM, but
avidity and intra resin exchange and recapture keep the bulk of protein
trapped in the resin).
Good luck!
Artem
On Tue, Oct 31, 2023, 3:21 PM Rafael Marques
wrote:
> Hi everyone,
>
> I have been looking on this bb and other websites as well bu
a Sci.
one above) because sugars in the medium react with amino acids resulting in
Brown Evil. Look it up if you're curious...
In recognition of the original author, references to Studier papers:
2005: https://pubmed.ncbi.nlm.nih.gov/15915565/
2014: https://pubmed.ncbi.nlm.nih.gov/2
Dear Jinhui Dong
You can simply make swimming-pool size amounts of it, as long as you have
basic lab equipment and supplies including an autoclave, and access to ~8
chemicals. The recipe is in the Studier papers, but I am happy to share it
with you here if you prefer.
Artem
- Cosmic Cats
Take a protein structure with waters, replace surface waters with Cu++ and
run some very basic MD to optimize distances. Many of the atoms will move
since Cu++ will find itself in an 'unfriendly' environment, as always
caveat emptor :)
Artem
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On F
With 50 mM Zn++ in solution, whatever it is will probably have a Zn salt in
it. So if you wanted to solve it by direct methods or via SAD - that should
do well. Sadly (hur hur) it's probably quite small, whatever it is.
Artem
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On Fri, Feb 3, 2023 at 6:
ce,
like a signal on a CAN bus or god knows what else.
All the best,
Artem
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On Fri, Feb 3, 2023 at 2:16 AM Tim Gruene wrote:
> Hi Artem,
>
> the simulator is exactly what I was looking for - many thanks!
> We did build a small circuit to generate
purchase a thermocouple simulator
https://www.brightwinelectronics.com/product/temperature-calibrator-k-n-thermocouple-generator-simulator
Artem
On Thu, Feb 2, 2023, 3:42 PM Rajkovic, Ivan <
95c2dc0d4fa4-dmarc-requ...@jiscmail.ac.uk> wrote:
> Hi Tim,
>
> Not sure if this woul
Hi Yong Tang,
I am not sure if there is another SER server out there, but if you can
share the sequence in a private email I can run it through my code that
does a similar type of analysis (it's not user friendly so I never did the
right thing and put it up as a server).
All the best,
One final comment: if you need orthogonal proteases, TEV and TVMV do not
seem to cut each others' sites (at least for me they don't). No idea if 3C
will cut both, or only TEV site.
Artem
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On Thu, Dec 8, 2022 at 5:26 AM Dom Bellini - MRC LMB <
Hi everyone,
The deadline to submit a screening proposal to the LCLS XFEL has been
extended! For delicate crystals and/or for crystals available in lower
quantities, the standard goniometer setup at LCLS-MFX is a useful option
available to all general users applying for beamtime.
See below for m
Dear CCP4-ers,
We're hiring :) In case you're interested, please visit the link below.
http://careers.animol-discovery.com/apply/fUyc5HcbL0/Head-Of-Medicinal-Chemistry?referrer=2022083118532124ZTDOKKYYDQHFBP
Artem
VP@ Animol
Cheesecake
g? If enough people share (even individual schedules) I am
happy to post the resulting schedule back here for everyone to enjoy...
Many thanks!
Artem
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To unsubscribe from the CCP4BB
ntisense.
Good luck!
Artem
P.S. if you use 2% glucose in the media be prepared to fight acidification
and the accumulation of Acetate in the spent medium, both of which can be a
problem - sometimes severe - for 'weaker' E. coli strains. Strongly
buffered medium is a must.
- Cosmic Cats ap
Yes we chose the sites based on predicted or known structure, to be sure.
No that was not a published thing, curse of commercial work :(
Happy to help on a private channel.
Artem
On Sat, Feb 19, 2022, 1:42 PM Tanner, John J. wrote:
> Casper: You make a good point about using 205 nm. I chec
many other options but their application is best considered with
more information in hand
Artem
On Fri, Feb 18, 2022, 8:02 PM Tanner, John J. wrote:
> Dear CCP4BB,
>
> We are working on a protein that has no Trp residues, which makes
> chromatography challenging due to the low absor
week only.
As to yield - it is very protein specific and you do get many variables in
play including promoters, cell lines, media additives and so on
Artem
On Mon, Feb 7, 2022, 11:25 AM Srivastava, Dhiraj <
dhiraj-srivast...@uiowa.edu> wrote:
> Hi All
> sorry for the question
specially if one requires near-complete passivity towards
strongly alkaline solutions.
Artem
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On Sun, Jan 30, 2022 at 1:02 PM Edward Berry wrote:
> After using the same reagents for the Lowry assay and seeing the color
> yield in the standard curve gradua
ector, Biochemistry
https://www.roivantcareers.com/job/associate-director-biochemistry-research-development-us-ma-boston-451-d-street-33-2057292/
Artem, the washer of many bottles (and also VP of biochem/biophys/structure
and hit finding)
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https://boards.greenhouse.io/roivantsciences/jobs/3549863
Finally posted! Thank you for looking.
Artem
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https
,
sex, national origin, disability status, genetics, protected veteran
status, sexual orientation, gender identity or expression, or any other
characteristic protected by federal, state or local laws.
Thank you,
Artem
(VP of stuff @ Ro
The Structural Molecular Biology (SMB) Division at the Stanford Synchrotron
Radiation Lightsource (SSRL) is seeking a Research Associate with a strong
background in X-ray diffraction data processing and/or software
development. A successful candidate will work with an interdisciplinary
group of cry
purify your
material. If your RNA is single stranded, it will have a hard time
surviving this ordeal. Of it is double stranded, should be no worries.
Artem
On Mon, Jun 21, 2021, 8:47 PM WENHE ZHONG
wrote:
> Dear members,
>
> We are planing to purify RNA only or/and RNA-protein comple
y when you add more substrate than enzyme the monomers of enzyme can
become 'smeared' along the DNA and this results in very odd kinetic
behavior.
Artem
On Fri, Jun 18, 2021, 12:05 AM Prem Prakash wrote:
> Dear all,
> Sorry for this off topic. I am working on an enzyme that has
s blue 2,6-dichloroindophenol into colorless reduced form
that spontaneously reoxidizes back to blue in air
And many others :)
Artem
On Wed, Jun 16, 2021, 6:19 PM P. H wrote:
> Hello All,
>
> We are looking for some candidate proteins for an undergraduate level
> advanced biochemist
Frontier
Pretty good service, but definitely not the cheapest.
Artem
On Tue, Jun 1, 2021, 4:54 AM Wim Zhong wrote:
> Hi Group,
>
> Does anyone have experience with CROs that can provide good compound
> service management in terms of quality and cost? We are looking for a
> pa
The Structural Molecular Biology (SMB) Division at the Stanford Synchrotron
Radiation Lightsource (SSRL) is seeking a Research Associate with a strong
background in X-ray diffraction data processing and/or software
development. A successful candidate will work with an interdisciplinary
group of cry
omain that
simply did not like the buffer conditions and was essentially insoluble
after cleavage due to its bull properties' mismatch with those of the
solvent.
Artem
On Mon, Mar 15, 2021, 5:08 AM Schreuder, Herman /DE <
herman.schreu...@sanofi.com> wrote:
> Dear Bulletin Board,
&g
4country%3DUnited+States&location_type=city&location_text=Boston%2C+MA%2C+United+States&location_autocomplete=true&radius=320
Artem
Silicon Therapeutics (“SITX”) is a privately held, physics-driven
integrated drug discovery company. SITX’s discovery efforts as well as
physic
quantify it by
dilution, transformation, and colony counting against a known standard
plasmid.
Happy new year
Artem
On Wed, Dec 30, 2020, 4:35 AM Anamika Singh wrote:
> Hi All,
>
> I have two constructs having different ori, p15ori and M13 ori, different
> promoters araBAD promoter a
Probably a good idea to share an image :) worth many words...
Artem
On Wed, Dec 9, 2020, 9:17 AM wrote:
> Dear All
> There is a 43kd protein purified via Ni-chelating affinity
> chromatography, anion exchange chromatography and gel filtration
> chromatography in sequence.
t, I guess I am just saying that literature, IMO, has long
ago stopped being generally directly reproducible. Not getting into the
obvious reasons as to why it happened, but still sad that it happened.
Artem
On Tue, Dec 8, 2020, 8:28 AM Hughes, Jonathan <
jon.hug...@bot3.bio.uni-giessen.de> wro
free to write directly for/with additional details.
Artem
On Mon, Oct 19, 2020, 3:18 AM Chiara Bruckmann
wrote:
> Dear all,
>
> Sorry for the off-topic question. I need to prepare an heterodimeric
> protein complex for an immunisation, and I would like to make sure that the
> dimer
Dear CCP4 friends,
I would like to let you know that we have just posted three jobs open
within my team at Silicon Therapeutics. Qualified inquiries are welcome!
Artem
1. Senior position in Biochemistry
https://silicontx.com/careers/open-positions/senior-investigator-principal-investigator
would like to gather some new ones especially if
people used them and liked the net result. Would be happy to summarize the
outcome and distribute the summary in this list.
Many thanks,
Artem
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will post it here.
Cheers,
Artem
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On Fri, Sep 11, 2020 at 10:26 AM Artem Evdokimov
wrote:
> Dear CCP4ers!
>
> I would like to once again benefit from the communal wisdom and experience
> of our community. Thank you in advance for your advice!
>
(assuming
there are enough replies to merit a summary).
Many thanks!
Artem
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t;> Modified version exit status 1
>>
>> -- after some digging I found several old, unsolved issues about that in
>> GitHub, any ideas about how to get around?
>>
>>
>>
>> ------
>>
>> To unsubscribe from t
ntent/285/18/13349.full.pdf
Artem
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On Tue, Aug 4, 2020 at 7:11 AM Panne, Daniel (Prof.) <
daniel.pa...@leicester.ac.uk> wrote:
> Hi Meytal,
>
> 1. The concentration seems way too high at 254mg/ml. Typically 0.1-5mg/ml
> are used.
>
Yes
It requires electroporation and very careful handling of the bacmid. Other
than that it is a fairly simple process.
Here is an example reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067234/
Artem
On Sat, Jul 18, 2020, 9:12 PM Digant Nayak wrote:
> Dear all,
>
> Sorr
with us the more we may be able to help you.
Artem
On Sat, Jun 27, 2020, 3:15 AM Umar Farook wrote:
> Dear All,
>
> Sorry for an offtopic question, your suggestions are highly appreciated.
>
> We have been working on iron sulfur cluster binding protein, which is
> usually expr
d their colleagues.”
*Verb*
“To discredit or invalidate an otherwise beautiful theory”
Artem
Artem
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On Tue, Jun 9, 2020 at 9:36 AM Tobias Beck wrote:
> Dear all,
>
> I was asked by a student what the highest resolution is, for each of the
> fo
dot
SHELX cup, it's one of my more treasured curios).
Artem
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On Mon, Jun 1, 2020 at 6:01 PM Jiyuan Ke
wrote:
> Hi Everyone,
>
> I want to crystallize a small organic molecule. I have very limited
> experience in small molecule crystallograph
Artificial helix bundles
GS
SUMO
Ubq
NusA
Fc and other mAb domains
Nanobodies (as fusions)
Cyt b562
Rubredoxin
Flavodoxin
VLR
Barnase
MHC proteins
Bostjan Kobe wrote a paper in 2015 which you probably saw already.
Artem
On Tue, May 5, 2020, 2:45 PM Murpholino Peligro
wrote:
> I was wonder
Rosetta will do this.
But it's not a server, and there is a fairly steep learning curve.
Artem
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On Mon, May 4, 2020 at 6:04 AM Firdous Tarique
wrote:
> Hi
>
> I am trying to incorporate novel disulfide bond by introducing Cys
> mutants
Thank you
Artem
On Tue, Apr 28, 2020, 7:22 PM Thomas Cleveland
wrote:
> Hi,
>
> There are tons of these listed on eBay, many in very good condition or
> even unused. I used to get them there all the time.
>
> Best
>
> On Tue, Apr 28, 2020 at 8:51 AM Artem Evdokimov
&g
Thank you very much!
Artem
On Tue, Apr 28, 2020, 1:03 PM Guenter Fritz <
guenter.fritz.phenix.c...@gmail.com> wrote:
> Dear Artem,
>
> I am a fan of the former "Superformance" column from Merck. The production
> / selling of these columns is sourced out to a small
deal. I was already looking at
Bio-Rad and found them almost as expensive, so I am actually looking for
some sort of 'knockoff' manufacturer :)
Thank you
Artem
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Gentle spin followed by TFF - a cheap peristaltic pump plus $200 filter
cartridge will do the trick.
Artem
On Mon, Apr 20, 2020, 11:56 AM Gloria Borgstahl
wrote:
> Hi Friends, We are secreting Spike Ecto domain into the media from insect
> cells for purification. As we scale u
cases like this, I normally choose not to publish, but maybe their
bosses insisted. Anyway, many options to find excuses, but it looks like
Artem has to go solve this structure himself.
Thank you all very much, many good responses :)
Arte,
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On Tue, Apr 7, 202
u're working on this,
which is not necessarily great since you're competing), or to just buck up
and do the structure on your own (which feels a bit wasteful). Then, you
realize that your friends at CCP4 have a lot of wisdom to offer, so you sit
down and pen an
>
https://www.finddx.org/covid-19/pipeline/
May be this saves someone twenty minutes of searching online.
Artem
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https://www.jis
equipment that may be available in good (sadly, not
all) high school labs.
Artem
On Thu, Apr 2, 2020, 3:17 PM Jurgen Bosch wrote:
> Hi Artem,
>
> How would you do this in resource limited countries?
> I think James idea is pointing toward that direction, deliver an easy to
> appl
human IgG and IgM antibodies, label them a
bit and do Homebrew ELISA on serum.
Artem
Artem
On Thu, Apr 2, 2020, 11:28 AM James Holton wrote:
> Personally, if I were infected with SARS-CoV-1 instead of SARS-CoV-2 I'd
> still like to know that.
>
> It is most certainly true that
n more, informally - please feel free to write me
directly
(2) if you'd like to apply, please write to *care...@enkochem.com*
Artem
VP of Biochemistry/Molecular Biology
Enko
To unsubscribe from the CCP4BB list,
they did, this is a nice product and it ought
to be a good money-maker for them)
Artem
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On Sun, Mar 15, 2020 at 5:27 PM Lau Kelvin wrote:
> Dear Artem,
>
>
> By work poorly do you mean, not as well and are you referring to all Expi
> cells?
>
ability to use the L-SeMet pure as opposed to the more common
L,D-mix then you can put in more. Either way, it works.
Expect your yield to suffer.
Question: did you try iodine incorporation? You can do it on the level of
pure protein, or crystals...
Artem
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short
helix.
Nature is cruel, but interesting.
Artem
On Fri, Jan 31, 2020, 10:33 AM Kluenemann, Thomas <
thomas.kluenem...@helmholtz-hzi.de> wrote:
> Dear all,
>
>
>
> We recently solved a the structure of a small c-type cytochrome. We
> observed, that of the eleven chain
then to crash the
aggregate out. This is of course somewhat extreme and cannot be recommended
as a general solution to every issue of this sort.
Addition of the right detergent can also help. Separation via another
orthogonal matrix is often helpful as well.
Artem
On Sun, Feb 2, 2020, 4:22 AM
.
Artem
On Sat, Feb 1, 2020, 6:22 PM Jon Hughes
wrote:
> Hello everyone,
> We work on a protein that tends to aggregate. The process is slowed but not
> stopped by glycerol and NDSB201. Interestingly, whereas guanidinium/HCl
> dissolves the aggregate readily, urea just turns it into
I bought a bunch of cheap cameras from Amscope. They were OK for the price.
Artem
On Wed, Nov 27, 2019, 12:46 Dean Derbyshire
wrote:
> Forgive the off topic subject:
>
>
>
> Has anyone got any experience with moticam microscope cameras?
>
> We are looking into cheap camer
FliS and FliC
Artem
On Sun, Nov 10, 2019, 03:42 Gianluca Cioci wrote:
> ps: the 3D structure
> of the complex should
> be solved at high resolution
>
> ;o)
>
>
>
>
> Dear All,
>
> I am looking for examples of two small proteins A and B that can form
machinery (any
personal impressions of these and any other similar LH devices are very
welcome!). I would be happy to post a condensed summary of replies.
Thank you
Artem
To unsubscribe from the CCP4BB list, click the following
I am willing to bet that the ketone is covalently reacting with the
arginine and the result is what you see :)
Artem
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On Thu, Nov 7, 2019 at 3:33 AM wrote:
> Dear HK,
>
> In your case mass spec would be extremely valuable. Not only could it
&
It happens.
Ef-Tu is methylated on a single lysine
Also LafV is a putative methylase in E. Coli but its effects were not
studied in detail.
Salmonella methylates flagellin which is how lysine methylation was
discovered - and E.coli is a kissing cousin so...
Artem
On Tue, Nov 5, 2019, 12:31
other methods)
Overall the key component to these methods is your ability to displace the
sodium with something else prior to measuring the effect of titration the
ion back. Isotopic Na is easier in this regard - but at a cost...
Hope this helps.
Artem
https://bmcresnotes.biomedcentral.com/articles
d together in a single
tube with air trapped between them - if the 'vent' is blocked (by e.g. old
grease or something) then these may pop.
https://www.olympus-lifescience.com/en/microscope-resource/primer/anatomy/oculars/
But the short version is right 99% of the time.
Artem
*"Pr
Unlimited length in an hour is physically unlikely, but the rest is not
that far off I think.
We are at or below 1 cent per basepair already (in bulk)...
Artem
On Wed, Jul 24, 2019, 13:01 Keller, Jacob wrote:
> Yes, imagine 1 hr, tabletop, unlimited-length, error-free, dirt-cheap g
Good morning,
We have two relevant job postingings that I would like to share.
Thank you for your attention :)
Artem
https://careers.massbio.org/job/sr-scientistprincipal-scientist-computational-chemistry/49455467/
and
https://careers.massbio.org/job/scientist-enzymology-protein-chemistry
s a bit of a distraction
from James' original point. However it's a valid opportunity for a lively
discussion on its own :)
Artem
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On Sun, Jul 21, 2019 at 4:52 PM Kay Diederichs <
kay.diederi...@uni-konstanz.de> wrote:
> Dear Artem
oblem #1 with this attitude (and an equivalent
of a very large army's worth in funding) then it can be solved.
Artem
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On Sun, Jul 21, 2019 at 1:55 PM Kay Diederichs <
kay.diederi...@uni-konstanz.de> wrote:
> Hi Artem,
>
> you are certain
predictable and artful phenomenon while
literally all other aspects of structure determination process (the gene to
structure pipeline, whatever you might call it)have made astronomic leaps
forward.
Artem
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On Mon, Jul 15, 2019 at 3:44 PM Holton, James M <
ob/plant-biology-research-assistant/48155606/>
- Patent Liaison
<https://careers.massbio.org/job/patent-liaison/48155601/>
(all the links above are from MassBio website and can be separately located
by searching for 'Enko')
Please direct inquiries/submissions to care...@en
Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact
points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing
.
Precipitation of protein prior to the gel is certainly an option, although
some proteins have difficultues with recovering after that (membrane
proteins in particular).
Artem
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On Sat, Mar 2, 2019 at 9:53 PM Keller, Jacob
wrote:
> Dear crystallographers,
>
>
&
high salt, and often
would do better in sodium acetate as opposed to chloride. I've worked with
ion channels that preferred 3M NaCl, or 2M MgCl2 and other fairly weird
conditions...
Artem
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On Fri, Mar 1, 2019 at 7:27 AM Alex Perálvarez Marín
wrote:
>
Dry shippers are often featured on auction sites for used equipment and
sometimes can be found for huge discounts...
Artem
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On Fri, Jan 25, 2019 at 8:36 AM Muhammad Imran wrote:
> Dear Community members,
>
> We wanted to buy molecular dime
)
Ferredoxin
Rubredoxin
cytochrome b562
Ubiquitin
the list goes on and on...
As to polycistronic stuff - it may be easier to mix lysates than to mess
around with multiple expression cassettes - in case you'd want to change
the mix later... just my two cents.
Artem
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ronment
I am probably missing another 10-15 methods but gotta run :)
Artem
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On Wed, Jan 2, 2019 at 2:42 PM Chandramohan Kattamuri <
1c5b7cb6c764-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Dear CCP4 members, Can someone suggest to me a met
My apologies for posting this on an open board :) I realize that this is
completely off topic - but it is a pretty painful topic that affects us all
regardless of academic or industrial affiliation, professional preferences
etc.
Artem
--- do not read below unless you want to :) ---
If an
https://www.unboundworlds.com/wp-content/uploads/2016/05/takei-oh-my.jpg
oh, my...
Happy new year!
Artem
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On Tue, Jan 1, 2019 at 5:38 PM Marin van Heel <
057a89ab08a1-dmarc-requ...@jiscmail.ac.uk> wrote:
> 1) I shall not make false FSC r
is sort of like a large 3D printer chassis with a
deck and pipettors bolted on. If you love to tinker this is a machine for
you. Cannot beat the price.
Artem
On Mon, Dec 31, 2018, 12:46 Doug Juers Hello All,
>
> I've just learned about the opentrons pipetting robot, which appears to
t EDTA, its complex with Zn is far too stable) and then add it directly
to your protein - note that adding Zn to protein directly tends to cause
precipitation (but chelating the Zn with a moderate-strength agent tends to
prevent this).
Good luck,
Artem
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