Gentle spin followed by TFF - a cheap peristaltic pump plus $200 filter
cartridge will do the trick.

Artem

On Mon, Apr 20, 2020, 11:56 AM Gloria Borgstahl <gborgst...@gmail.com>
wrote:

> Hi Friends,  We are secreting Spike Ecto domain into the media from insect
> cells for purification.  As we scale up I am wondering what is recommended
> for collecting the media from large volumes of culture.  Centrifugation?
> Filtration of some kind?  I imagine we need to be gentle to not lyse the
> insect cells.  Thank you, Gloria
>
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