Gentle spin followed by TFF - a cheap peristaltic pump plus $200 filter cartridge will do the trick.
Artem On Mon, Apr 20, 2020, 11:56 AM Gloria Borgstahl <gborgst...@gmail.com> wrote: > Hi Friends, We are secreting Spike Ecto domain into the media from insect > cells for purification. As we scale up I am wondering what is recommended > for collecting the media from large volumes of culture. Centrifugation? > Filtration of some kind? I imagine we need to be gentle to not lyse the > insect cells. Thank you, Gloria > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1