Could be dna crystals. They often do not diffract. Artem
On Wed, Nov 8, 2023, 11:18 AM careinaedgo...@yahoo.com < 000002531c126adf-dmarc-requ...@jiscmail.ac.uk> wrote: > > The reservior solution is 0.2 M NaCl2, 0.1 M HEPES pH 7.5, and 25% 3350 PEG > > Protein buffer is 300 mM NaCl, 20 mM HEPES pH 7.5, 3mM TCEP > > The drop contains 1.5ul protein-DNA complex and 1.5 ul reservior solution > > > On Wednesday, November 8, 2023 at 05:12:25 PM GMT+2, > stephen.c...@rc-harwell.ac.uk <stephen.c...@rc-harwell.ac.uk> wrote: > > > Hi Careina, > > Without knowing what's in your protein buffer or crystallisation condition > it's hard to comment. > > Best wishes, > Steve > > Dr Stephen Carr > Research Complex at Harwell (RCaH) > Rutherford Appleton Laboratory > Harwell Oxford > Didcot > Oxon OX11 0FA > United Kingdom > Email stephen.c...@rc-harwell.ac.uk > tel 01235 567717 > ------------------------------ > *From:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of > careinaedgo...@yahoo.com <000002531c126adf-dmarc-requ...@jiscmail.ac.uk> > *Sent:* 08 November 2023 15:00 > *To:* ccp4bb <ccp4bb@jiscmail.ac.uk> > *Subject:* [ccp4bb] What could these crystals be? > > Hi all > We have been trying with no success to crystalize a protein. Recently we > got these strange shape "crystals". They are hard and flat but they do not > diffract at all. Any ideas as to what could cause this? > Careina > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This email and any attachments may contain confidential, copyrightand or > privileged material, and are for the use of the intended addressee only. If > you are not the intended addressee or an authorized recipient of the > addressee, please notify us of receipt by returning the e-mail and do not > use, copy, retain, distribute or disclose the information in or attached to > this email. > > > > Any views or opinions presented are solely those of the author and do not > necessarily represent those of the Research Complex at Harwell. > > > > There is no guarantee that this email or any attachments are free from > viruses and we cannot accept liability for any damage which you may sustain > as a result of software viruses which may be transmitted in or with the > message. > > > > We use an electronic filing system. Please send electronic versions of > documents, unless paper is specifically requested. > > > > This email may have a protective marking, for an explanation, please see: > > > http://www.mrc.ac.uk/About/informationandstandards/documentmarking/index.htm > . > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
