Neat! Looks like multiple adjacent bubbles that were initially touching but eventually shrunk down to the central cores - the connectors are protein filaments (skin on the bubbles) left over from when bubbles had contact points.
Artem On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) <bevan.marsh...@csiro.au> wrote: > Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that > condition is found in both Index and JCSG screens as well as Classics II. > > > > > > *Bevan Marshall* > Staff Scientist | Collaborative Crystallisation Centre > *Manufacturing* > CSIRO > > E bevan.marsh...@csiro.aut +61 3 9662 7492 > 343-351 Royal Parade, Parkville, VIC 3052 > www.csiro.au | https://c3.csiro.au > CSIRO acknowledges the Traditional Owners of the lands that we live and > work on across Australia and pays its respect to Elders past and present. > > *PLEASE NOTE* > The information contained in this email may be confidential or privileged. > Any unauthorised use or disclosure is prohibited. If you have received this > email in error, please delete it immediately and notify the sender by > return email. Thank you. To the extent permitted by law, CSIRO does not > represent, warrant and/or guarantee that the integrity of this > communication has been maintained or that the communication is free of > errors, virus, interception or interference. > > *Please consider the environment before printing this email.* > > > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *LEGRAND > Pierre > *Sent:* Thursday, 28 March 2019 9:13 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* Re: [ccp4bb] Interesting pattern on a crystallization drop > > > > Dear Beatriz, > > > > Nice drops :-)) > > Could it be that there is a reaction going on in these drops ? > > The conditions are quite "exotic" with possibilities of coordination or > oxydoreduction (Co2+/Co3+) or polymerization... > > Do you have reductants with the protein buffer ? > > Is the protein an enzyme or a metalloprotein ? > > Just some ideas. > > > > Best wishes, > > Pierre > > > ------------------------------ > > *De :* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz > Gomes Guimaraes [beatriz.guimar...@fiocruz.br] > *Envoyé :* mercredi 27 mars 2019 19:44 > *À :* CCP4BB@JISCMAIL.AC.UK > *Objet :* [ccp4bb] Interesting pattern on a crystallization drop > > Dear all, > > > > I would like to share with you a surprising pattern I found when examining > some crystallization plates (attached figures). > > > > It is less obvious looking the photos, but apparently the "lines" are > formed by precipitated protein and there are some "bubbles" with small > drops inside. I wish they were microcrystals but I do not think this is > the case. > > I was suprised by the symmetry ! > > > > And it is not completely random because for the same condition the > difference between the two drops are : protein alone ("hexagon") and > protein + ligand ("rhombus") > > > > crystallization condition is: > > 0.01 M Cobalt(II) chloride hexahydrate > > 0.1 M Tris pH 8.5 > > 20% w/v Polyvinylpyrrolidone K 15 > > > > Have you seen anything similar before? > > > > Thank you for your comments! > > Beatriz > > > > > > -------------------------- > Beatriz Guimarães > Laboratory of Structural Biology and Protein Engineering > Instituto Carlos Chagas - ICC / FIOCRUZ Paraná > Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C > CIC 81350-010 > Curitiba - PR, Brasil > Tel.:+55(41)3316-3225/2104-3438 > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
