If you have hopes for cysteine residues in reasonable proximity, then bis-iodoacetamide (with a suitable spacer, commercially available is the ethylenediamine spacer). Any reasonable chemist can make you other spacer lengths to order.
Homobifunctional PEG with maleimide, N-hydroxy succinimide esters, etc might work also. Homobifunctional PEG with amines on ends can be used with a water soluble coupler e.g. EDC NBT mix. Notably if you are lucky just treating protein as a dimer with EDC NBT mix can promote clinking. Bis aldehydes are an old standby but they are usually quite harsh. Feel free to write directly for/with additional details. Artem On Mon, Oct 19, 2020, 3:18 AM Chiara Bruckmann <bruckmannchi...@gmail.com> wrote: > Dear all, > > Sorry for the off-topic question. I need to prepare an heterodimeric > protein complex for an immunisation, and I would like to make sure that the > dimer will be stable and it won't dissociate after injection. > > I am wondering if any of you has a mild cross-linking protocol (and > suggestions of a suitable reagent) to share. > > Thanks and regards, > Chiara > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
