Affirmative on all these questions:

SUMO works well
SUMO protease is super easy to make in house

As to which one - regular SUMO for E. coli and SUMO* for mammalian or
insect cell systems because regular SUMO may get cleaved by the intrinsic
protease. SUMO* is a triple mutant, with corresponding mutations in the
protease, such that the native protease does not cleave any more.

Happy to help further via PM.

Artem


On Thu, Dec 12, 2024, 11:36 Gloria Borgstahl <gborgst...@gmail.com> wrote:

> Hello friends, Yet another kinda off topic question from me.  I am looking
> into SUMO tags for my protein of interest to help solubility.   Has this
> worked for you?  If so which SUMO and protease do you use?  Can the
> protease be made in house?  So far I am looking at the pET SUMO system.  Is
> this the best?  Thank you in advance. Gloria
>
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