Affirmative on all these questions: SUMO works well SUMO protease is super easy to make in house
As to which one - regular SUMO for E. coli and SUMO* for mammalian or insect cell systems because regular SUMO may get cleaved by the intrinsic protease. SUMO* is a triple mutant, with corresponding mutations in the protease, such that the native protease does not cleave any more. Happy to help further via PM. Artem On Thu, Dec 12, 2024, 11:36 Gloria Borgstahl <gborgst...@gmail.com> wrote: > Hello friends, Yet another kinda off topic question from me. I am looking > into SUMO tags for my protein of interest to help solubility. Has this > worked for you? If so which SUMO and protease do you use? Can the > protease be made in house? So far I am looking at the pET SUMO system. Is > this the best? Thank you in advance. Gloria > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
