[ccp4bb] Investigator position at the Hauptman-Woodward medical research Institute in Buffalo, New York

ctoral opportunity, is available at https://hwi.buffalo.edu/about-us/careers/. Sincerely, Eddie Snell Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center Professor, Materials Design and Innovation | Univers

[ccp4bb] The 2023 International BioXFEL (Biological applications of X-ray Free Electron lasers) Conference, May 16th-18th (Workshop May 15th) San Juan Puerto Rico

ws network and further their opportunities. Best, Eddie Snell Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center Professor, Materials Design and Innovation | University at Buffalo, SUNY Fellow of th

[ccp4bb] Off topic - 3D models demonstrating drug binding to students

experiences with this nature of teaching, any offline feedback would be appreciated. Thank you, Eddie Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center Professor, Materials Design and Innovation | University

[ccp4bb] Position available - Established investigator - structural biology of age related diseases

ctions. Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center BioInnovations Chaired Professorship | University at Buffalo, SUNY Fellow of the American Crystallographic Society (The Structural Science Society) p:

Re: [ccp4bb] Crystallization Robots

near future to celebrate over two decades of operations. Best, Eddie Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center Professor, Materials Design and Innovation | University at Buffalo, SUNY p: +1 716 898 8

Re: [ccp4bb] Has anyone successfully used RoseTTAFold or AF2 to guide crystallization?

biology resources page - Structural Biology Resources | Hauptman-Woodward Medical Research Institute (buffalo.edu)<https://hwi.buffalo.edu/structural-biology-resources/>. Best, Eddie Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF B

[ccp4bb] Cryo-EM Center Director Opportunity

State and Federal laws, or any other legally protected characteristics. This policy applies to all conditions and terms of employment. Please pass on these details to anyone who may be interested. Sincerely, Eddie Snell Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical

Re: [ccp4bb] sugestions on weak diffracting protein crystals

complex, but also seen to be successful in cases, is altering the humidity of the crystals. There are commercial devices to do this and beamlines equipped with humidity control. This is a more technically challenging approach. Good luck, Eddie Edward Snell Ph.D. President and CEO | Hauptman

[ccp4bb] Cryo-EM data processing specialist at the Hauptman-Woodward Medical Research Institute

Jiang, or by mail to: 700 Ellicott Street, Buffalo, NY 14203, USA. Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center Professor, Materias Design and Innovation | University at Buffalo, SUNY p: +1 716 898 863

Re: [ccp4bb] Elemental Analysis

at produce a model of the actual ligand that was present. Best, Eddie https://getacrystal.org - The High-throughput Crystallization Screening Center Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center BioI

[ccp4bb] Research scientist position to support data processing for XFEL experiments with the NSF BioXFEL Science and Technology Center

A research scientist is sought to join the BioXFEL team. BioXFEL<https://www.bioxfel.org/> is an NSF Supported Science and Technology Center developing the biological applications of X-ray Free Electron Lasers. It is a consortium of 8 institutions directed by Dr. Edward Snell throu

[ccp4bb] Postdoctoral position in the Snell/Bowman laboratories at the Hauptman-Woodward Medical Research Institute

llization screening of biological macromolecules. Sincerely, Edward Snell Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center BioInnovations Chaired Professorship | University at Buffalo, SUNY p: +1 716 89

Re: [ccp4bb] Quote source inquiry

ievably we were able to collect our data and get a publication from it. Best, Eddie Edward Snell Ph.D. Director of the NSF BioXFEL Science and Technology Center President and CEO Hauptman-Woodward Medical Research Institute BioInnovations Chaired Professorship, University at Buffalo, SUNY 700

Re: [ccp4bb] Highest resolution of X-ray / neutron / electron crystallography, cryo EM

no need to push the data, risk radiation chemistry impacting the interpretation, and spending months of computing time to try to explain every niggly detail that pops up ☺ Best, Eddie Edward Snell Ph.D. Director of the NSF BioXFEL Science and Technology Center President and CEO Hauptman

Re: [ccp4bb] Strange Pseudosymmetry Effects

of the kinematical approximation versus application of dynamical theories? Certainly the computational power is available. Just my 2 cents for the day. Best, Eddie Edward Snell Ph.D. Director of the NSF BioXFEL Science and Technology Center President and CEO Hauptman-Woodward Medical

[ccp4bb] Crystallization and synchrotron resources available.

hoton Source, Argonne National Laboratory Vice President for Advancing Therapeutics Hauptman-Woodward Medical Research Institute Interim CEO American Crystallographic Association 630.252.0544 ke...@imca-cat.org Stay safe and healthy, Eddie ?Edward Snell Ph.D. Webpage: https://hwi.buffalo.e

[ccp4bb] Capturing the unique educational material currently being produced

Dear CCP4 members, I'm not sure what the situation is like in other countries but in the US while we try to minimize the spread of infection many of us in education are discovering the joys of Zoom and the like and making lectures, lessons, coursework etc. electronic. Within our community a va

Re: [ccp4bb] Paired refinement proves data quality goes beyond the spatial limits of the detector

We managed to remove the detector and consequently all the instrument noise using the same procedure. Remarkable effect - absolutely noiseless images. We call it the Adjusted Pixel Readout Index Linked effect. There must be an Ig prize in this. (shameless but serious plug - our Crystallization

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

?It is great that this workshop is occurring but I couldn't help but notice that there seem to be a lot of male speakers and tutors. I was wondering if it might be appropriate to add some female role models. There are some great candidates? From: CCP4 bulletin

Re: [ccp4bb] Protein concentration for the initial crystallisation trials

, may your New Year Resolutions be high. Best, Eddie Edward Snell Ph.D. Director of the NSF BioXFEL Science and Technology Center President and CEO Hauptman-Woodward Medical Research Institute BioInnovations Chaired Professorship, University at Buffalo, SUNY 700 Ellicott Street, Buffalo, NY

Re: [ccp4bb] how to get protein crystal

Dear Amala, There are some good suggestions coming your way from here. I would also recommend checking out the High-Throughput Crystallization Screening Center at the Hauptman-Woodward Medical Research Institute or other similar services. They run most of the common types of screens in an effi

Re: [ccp4bb] Importance of temperature during initial crystallization screening

these cases! I think a need to explore this would be some sort of temperature controlled harvesting box. If anyone out there knows of one, I have a project with a real need for this unrelated to the crystallization step. Best, Eddie Edward Snell Ph.D. Biological Small Angle Scattering The

Re: [ccp4bb] challenges in structural biology

for support in 2020. It really helps bring in great speakers, start many new collaborations, and grow the field. Best, Eddie /Lurk mode on Edward Snell Ph.D. Biological Small Angle Scattering Theory and Practice, Eaton E. Lattman, Thomas D. Grant, and Edward H. Snell. Available through all

[ccp4bb] Cryo-EM director position at the Hauptman-Woodward Medical Research Institute in Buffalo, NY.

careers<http://www.hwi.buffalo.edu/careers> Edward Snell Ph.D. Biological Small Angle Scattering Theory and Practice, Eaton E. Lattman, Thomas D. Grant, and Edward H. Snell. Available through all good bookshops, or direct from Oxford University Press Director of the NSF BioXFEL Science and

Re: [ccp4bb] change in unit cell volume

but that’s not to say that it doesn’t happen. Best, Eddie PS. Shameless plug –- http://getacrystal.com Edward Snell Ph.D. Biological Small Angle Scattering Theory and Practice, Eaton E. Lattman, Thomas D. Grant, and Edward H. Snell. Available through all good bookshops, or direct from Oxford

Re: [ccp4bb] Issue with high Rfree (0.25) for a high-resolution dataset (1.05 Ang)

s a decade ago and the structure is still being refined - such is the richness of high-resolution. If something diffracts like this, pull the detector back and ignore the temptation ☺ Best, Eddie Shameless plug – http://getacrystal.org Edward Snell Ph.D. Biological Small Angle Scattering Theory

Re: [ccp4bb] Should we still keep copies of all raw data?

this. I fully subscribe to the concept of storing raw images but there are very real practical and financial problems :( Cheers, Eddie Edward Snell Ph.D. Biological Small Angle Scattering Theory and Practice, Eaton E. Lattman, Thomas D. Grant, and Edward H. Snell. Available through all goo

Re: [ccp4bb] Please share your experience about "ugly" crystals showing good diffraction

. Best, Eddie Edward Snell Ph.D. Biological Small Angle Scattering Theory and Practice, Eaton E. Lattman, Thomas D. Grant, and Edward H. Snell. Available through all good bookshops, or direct from Oxford University Press Director of the NSF BioXFEL Science and Technology Center President and

Re: [ccp4bb] suggestion of crystallization optimization

very useful to identify other conditions that initially look like precipitate but that can be successfully optimized into well diffracting crystals. Best, Eddie Edward Snell Ph.D. Biological Small Angle Scattering Theory and Practice, Eaton E. Lattman, Thomas D. Grant, and Edward H. Snell. Avai

Re: [ccp4bb] Structure with missing density

some use to answering your question, Best wishes, Eddie Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute BioInnovations Chaired Professorship, University at Buffalo, SUNY 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu Phone: (716) 898 8631

Re: [ccp4bb] sending dewar to synchrotrons

still full with liquid nitrogen when improperly labeled carries a stiff penalty. Cheers, Eddie Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute BioInnovations Chaired Professorship, University at Buffalo, SUNY 700 Ellicott Street, Buffalo, NY 14203-1102

Re: [ccp4bb] ice build up during collection using 4 axis goniometer

this effectively. I may be 'misremembering' but I think James Holton at the ALS had a wonderful design and I am sure there are others out there. Ours is a Falcon tube with a handle and a small hole but as the saying goes, don't try that at home. Good luck, ice is the enemy, Edd

[ccp4bb] Off topic but of interest to SSRL users

providing guidelines on what the user community can do to educate Washington DC as to how important SSRL has been for career development, discovery, and the economy of the USA. In advance of that advice please visit the page and show your support. Thanks, Eddie Snell. Edward Snell Ph.D

Re: [ccp4bb] crystallization screen for protein-protein complex

screening center at our institute uses a lot of the commercially available screens (http://hwi.buffalo.edu/crystallization-cocktails/) and covers a fairly extensive area of chemical space. That can save a lot of time and effort. Details are at http://getacrystal.org. Good luck, Eddie Edward

Re: [ccp4bb] shadow at the edge

If it moves it’s the shadow of the Kappa head. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu Phone: (716) 898 8631

Re: [ccp4bb] Recommendations for Robotic Crystal Screening Services

uccessful crystallizations, Eddie. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu<http://hwi.buffalo.edu/> Phone: (716) 8

Re: [ccp4bb] Recommendations for Robotic Crystal Screening Services

works with a lot of laboratories. Cheers, Eddie. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu<http://hwi.buffalo.edu/>

Re: [ccp4bb] suggestion on protein crystallization optimization from phase separation

, Biophys J. 94, 570-583, 2008, 10.1529/biophysj.107.116152) can be recast in the conventional phase diagram space and shows how temperature affects the gelation point and can be used to shift the phase. Cheers, Eddie http://Getacrystal.org Edward Snell Ph.D. President and CEO Hauptman

[ccp4bb] Multiple positions in Buffalo NY

ations should be addressed to the emails given in the position descriptions. Happy New Year, Eddie Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102

Re: [ccp4bb] Shipping crystals for RT data collection

Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email

[ccp4bb] Scientist position with the Hauptman-Woodward High-Throughput Crystallization Center

alo.edu<mailto:esn...@hwi.buffalo.edu>. Formal applications should send a cover letter and resume along with three contacts for references to opportun...@hwi.buffalo.edu<mailto:opportun...@hwi.buffalo.edu>. The position is available in the New Year and will be open until filled. Edward Snell Ph

Re: [ccp4bb] Crystal with ZERO diffraction

ull of strongly diffracting crystals and plenty of X-rays. Best wishes, Eddie Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 http://hwi.bu

Re: [ccp4bb] Effects of Multiplicity and Fine Phi with Equivalent Count Numbers

Eddie. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell

Re: [ccp4bb] 5th Banff Meeting on Structural Dynamics, 18/19th Febr. - 22nd February 2017

?This looks like a very interesting scientific meeting but I would just like to point out an observation based on the current invited speakers and program committee - there are also scientists who are women in this field. Gentleman, a little bit of effort would go a long way. Apologies for m

Re: [ccp4bb] FW: [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

discussion board. I for one would like to keep it there. Political views aside, we’re all trying to achieve the same scientific goals. Let’s remember that and keep that the focus. Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural

[ccp4bb] Full professor position at the University of Buffalo in Biological Applications of X-ray Free Electron Lasers

that appointment in Physics or Structural Biology, for example, would be possible. Cheers, Eddie http://hwi.buffalo.edu The High-throughput Crystallization Center - Our expertise, Your results: http://hwi.buffalo.edu/science/high-throughput-crystallization-center/ Edward Snell Ph.D. Presid

Re: [ccp4bb] crystal mosaicity values

Edward Snell Ph.D. CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: esn

Re: [ccp4bb] crystal mosaicity values

not trivial. Cheers, Eddie GRC Diffraction Methods Conference - Deadline for registration approaching. https://www.grc.org/programs.aspx?id=11655 Edward Snell Ph.D. CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott

[ccp4bb] Position for a person experienced in structure based drug design.

d cover letter to: i...@harkerbio.com ------- Edward Snell Ph.D. CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, SUNY Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716)

[ccp4bb] Position for an experienced PhD-level structural biologist with sales and commercial experience

didate and circumstances. To Apply Please send a CV and cover letter to: i...@harkerbio.com ------- Edward Snell Ph.D. CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, SUNY Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 Phone:

Re: [ccp4bb] Salt!

the application of these two techniques in your case. If you don't have it available locally, while trying not to advertise, it is available to the community as a service - just don't ask me! The lab can be contacted directly at hts...@hwi.buffalo.edu<mailto:hts...@hwi.buffalo.e

Re: [ccp4bb] suggestions for cryoprotectant

You might get some clues from Acta Cryst. (2008). D64, 287-301[ doi:10.1107/S0907444907067613 ] Glycerol concentrations required for the successful vitrification of cocktail conditions in a high-throughput crystallization screen R. Kempkes, et al. and J. Appl. Cryst. (2002). 35, 538-545

[ccp4bb] Postdoctoral Researcher Opportunity in the application of free-electron lasers to structural biology.

-8597 email: jszcze...@hwi.buffalo.edu<mailto:jszcze...@hwi.buffalo.edu> Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 89

[ccp4bb] 71st Annual Pittsburgh Diffraction Conference

. For more information contact the organizers at pdiffract...@hwi.buffalo.edu Sincerely, Eddie Snell Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone

[ccp4bb] Of topic, trying to identify a reference for multiple crystal data collection

nce I'd be very grateful. If I should have read your book where it appeared I apologize! I doubt the board would be interested in this so please send me a reply offline. Thanks. Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Ha

Re: [ccp4bb] frosted crystals during storage in pucks

Ditto - I was always impressed with the contraption in the Garman lab which, if I remember correctly, is made of a thick block of wood and some plumbing pipes. It is designed to hold empty open Dewars inverted so they could dry. Edward Snell Ph.D. Assistant Prof. Department of Structural

Re: [ccp4bb] cryo condition

cryoprotectant including: Todd Holyoak, Timothy D. Fenn, Mark A. Wilson, Aaron G. Moulin, Dagmar Ringe and Gregory A. Petsko. (2003) Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. Acta D. 59, 2356-2358. Cheers, Eddie. Edward Snell Ph.D. Assistant

Re: [ccp4bb] saxs on xtals

the possibility of anomalous SAXS with and without doped ferritin? Note that you may be inducing interparticle effects which will complicate the interpretation of any SAXS data, solution or suspended crystals. Cheers,, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biolog

Re: [ccp4bb] No diffraction

And ambient if you were using cryo ... Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype

Re: [ccp4bb] live streaming of ccp4 study weekend

Hi Charles, For both the morning (after I got up) and afternoon session slides have been fine - no video or audio though. A connection to the server could not be established :( Had to update my RealPlayer to a Nov 2011 release before seeing anything. Cheers, Eddie Edward Snell Ph.D

Re: [ccp4bb] Same protein, different molecule numbers per ASU

his field and some of the points above. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660

Re: [ccp4bb] Phase Separation

4, 368-377. 3. Vekilov, P. G. Crystal Growth & Design 2004, 4, 671-685. 4. Bergfors, T. J Struct Biol 2003, 142, 66-76. 5. Dumetz, A. C.; Chockla, A. M.; Kaler, E. W.; Lenhoff, A. M. Biophys J 2008, 94, 570-583. Hope this helps, Cheers, Eddie Edward Snell Ph.D. Assistant Prof

Re: [ccp4bb] Saxs reviews or books

I'd also highly recommend X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution Christopher D. Putnam, Michal Hammel, Greg L. Hura and John A. Tainer Quarterly Reviews of Biophysics /

Re: [ccp4bb] pH dependent conformational change

SAXS, if the motion is not random and especially if you have crystallographic/NMR structure for the domains.. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203

Re: [ccp4bb] Strange spots

They remind me of the fiber bundle on optic taper between the phosphor and the CCD. That's from things I saw years ago (BC - before crystallography) and I don't know the system in use here. I suspect it's instrumental and any diffraction in that area of the detector should show similar effects

Re: [ccp4bb] **Possible spam**cryoprotetant for 35% Dioxane

. (2006). 39, 244-251[ doi:10.1107/S0021889806004717 ] Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions V. Berejnov, N. S. Husseini, O. A. Alsaied and R. E. Thorne Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology

[ccp4bb] Postdoc position at Hauptman-Woodward Medical Research Institute, Buffalo, New York

ntown Buffalo, NY, USA. Informal enquiries are welcome to either Eddie Snell (esn...@hwi.buffalo.edu) or Joseph Luft (l...@hwi.buiffalo.edu). To apply please send a current resume and the names of two references to Anne Kent, Human Resources Coordinator (k...@hwi.buffalo.edu). Thanks, Eddie. E

Re: [ccp4bb] Micro-g Crystal Growth and the literature

exploiting this but it could be argued that it is a much better investment overall to play with molecular biology techniques or a more brilliant beamline. However, if you are going to be in space anyway, it's a small mass, automated payload, potential high payoff etc... Cheers, Eddie Ed

[ccp4bb] I 1 21 1 and C1 21 1 in CCP4

with the obvious. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snel

Re: [ccp4bb] question on the effect of a fire alarm on crystallization

f the high-frequency effects. I would not worry about the effect of the fire alarm, just the process of putting it in! Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo

Re: [ccp4bb] Refining against images instead of only reflections

when the tape drives were slower writing data than the detectors producing it. That mad scramble to start backup before starting collection ;) Realtime readout, continuous rotation etc., may need to redefine our thoughts of images. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of

[ccp4bb] Off topic: Position announcement - Chair, Department of Structural and Computational Biology - SUNY Buffalo

cally to Dr. Bruce Holm, Chair Structural and Computational Biology Search Committee, bah...@buffalo.edu. The University at Buffalo is an Affirmative Action/Equal Opportunity Employer ---- Edward Snell Ph.D. Assistant Prof. Department of St

[ccp4bb] Off topic: Crystal degredation with age

or tell me if I'm imagining things? I've found plenty on the protein prep etc. but nothing on the crystal. Thanks, Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY

Re: [ccp4bb] dry shipper & FedEx

und them very helpful. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Email: esn...@hwi.buffalo.edu  Telep

Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer

systematic trials in comparison to more conventional spectrometers; especially concerning the accuracy and reproducibility. The ease of use was very nice but we did not buy one. Thanks, Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodwa

Re: [ccp4bb] Crystallographic computing platform recommendations?

ay to go. Has anyone had any experiences in transitioning from Fedora to Ubuntu? Is it something to think about, a Thanksgiving project mainly? Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute

Re: [ccp4bb] Are cell parameter shifts real?

the mosaicity of the crystal is masked by the contribution of the geometrical and spectral effects of the instrumentation and beam. This is especially the case on a lab source. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Haupt

Re: [ccp4bb] Spooky, moving crystals

m? Once cooled they should remain that way. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Email: [

Re: [ccp4bb] Coot and Hs

fering with hydrogen any fix would be appreciated. For now, I am pulling the detector back :-) Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716)

Re: [ccp4bb] poll: cutoff for "high resolution"

:) Cheers, Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Email: [EMAIL PROTECTED] Telepathy: 42.2 GHz Heis

Re: [ccp4bb] nature cb3 response

Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Email: [EMAIL PROTECTED] Telepathy: 42.2 GHz Heisenberg was pro

Re: [ccp4bb] Metal-ligand(s) positioning following refinement...

o the Mn4Ca complex in single crystals of photosystem II: A case study for metalloprotein crystallography. It's a good example of the problem. Other studies are out there with similar effects send for Fe and Cu. Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Bio

Re: [ccp4bb] Help with reducing crystal mosaicity

m temperature but at that resolution you are going to need all the X-rays you can get onto it. Cheers, Eddie. Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716)