Dear Hena, You already have excellent advice but I would also look at the PEG smear approach developed by Frank von Delft’s group – Chaikuad et al., Acta Cryst D. 7, 1627-39 (2015). It’s commercially marketed by Molecular Dimensions and has features which may be amenable to complex systems. Caveat emptor, we don’t have any data on any of the academic samples that have been submitted to our crystallization center to vouch for its success but we would suggest taking a look.
If you are interested in testing a lot of conditions in an efficient way the high-throughput crystallization screening center at our institute uses a lot of the commercially available screens (http://hwi.buffalo.edu/crystallization-cocktails/) and covers a fairly extensive area of chemical space. That can save a lot of time and effort. Details are at http://getacrystal.org. Good luck, Eddie Edward Snell Ph.D. President and CEO Hauptman-Woodward Medical Research Institute Assistant Prof. Department of Structural Biology, University at Buffalo 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: esn...@hwi.buffalo.edu [cid:image003.png@01D2D241.8F507E50] Heisenberg was probably here! From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hena Dutta Sent: Saturday, May 20, 2017 5:15 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] crystallization screen for protein-protein complex Dear Members, I am trying to crystallize a protein-protein complex. Both are soluble proteins and form complex as observed in FPLC either co-purifying or separately purifying and then mixing with equi-molar ratio. Looking for suggested screens to try for crystallization. So far tried with Qiagen - ProComplex Suite, Classics Suite and Classics II Suite No success yet. Is there any guidance which screen to try? Looking for your help... Regards, Hena.
