Not to forget J. Appl. Cryst. (1996). 29, 584-587 [ doi:10.1107/S0021889896004190 ] Glycerol concentrations required for cryoprotection of 50 typical protein crystallization solutions E. F. Garman and E. P. Mitchell
Which prompted the McFerrin and Snell work. Also worth checking out (and I apologize if I missed others) Acta Cryst. (2008). D64, 287-301 [ doi:10.1107/S0907444907067613 ] Glycerol concentrations required for the successful vitrification of cocktail conditions in a high-throughput crystallization screen R. Kempkes, E. Stofko, K. Lam and E. H. Snell J. Appl. Cryst. (2006). 39, 244-251 [ doi:10.1107/S0021889806004717 ] Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions V. Berejnov, N. S. Husseini, O. A. Alsaied and R. E. Thorne Cheers, Eddie Edward Snell Ph.D. Assistant Prof. Department of Structural Biology, SUNY Buffalo, Senior Scientist, Hauptman-Woodward Medical Research Institute 700 Ellicott Street, Buffalo, NY 14203-1102 Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: esn...@hwi.buffalo.edu Telepathy: 42.2 GHz Heisenberg was probably here! -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Eric Larson Sent: Thursday, October 21, 2010 12:14 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] **Possible spam**cryoprotetant for 35% Dioxane Hi Jerry, A great reference for initial cryocondition searches for many "standard" crystallization solutions is the tables in: J. Appl. Cryst. (2002). 35, 538-545 [ doi:10.1107/S0021889802009238 ] The development and application of a method to quantify the quality of cryoprotectant solutions using standard area-detector X-ray images M. B. McFerrin and E. H. Snell On the bottom of page 542 it says for 35% dioxane as the precipitant in Hampton Crystal Screen II condition # 4: 25% glycerol, 25% PEG 400, 20% ethylene glycol, 15% propylene glycol (1,2-propanediol) good luck, Eric __________________________ Eric Larson, PhD Biomolecular Structure Center Department of Biochemistry Box 357742 University of Washington Seattle, WA 98195 On Wed, 20 Oct 2010, Jerry McCully wrote: | Dear All; | | We just got some crystals from 35% (v/v) Dioxane. We are going to collect some data soon. | | Does anyone have the experience with the cryoprotectant in this condition? | | Thanks a lot, | | Jerry McCully | | | |
