I’m reminded of a comment Wim Hol made at an ACA meeting many years ago during a discussion of resolution. He noted that you had to add molecular weight into the equation also to really get a handle on how impressive an experiment is. Some proteins are larger small molecules, others are tour de force examples.
Having collected and still working on a 0.8A data set from a tetramer of a ~40kDa protein I advise my students only half-jokingly to move the detector back and pretend that didn’t see anything beyond 1A. If you can answer the question with the resolution you have then there is no need to push the data, risk radiation chemistry impacting the interpretation, and spending months of computing time to try to explain every niggly detail that pops up ☺ Best, Eddie Edward Snell Ph.D. Director of the NSF BioXFEL Science and Technology Center President and CEO Hauptman-Woodward Medical Research Institute BioInnovations Chaired Professorship, University at Buffalo, SUNY 700 Ellicott Street, Buffalo, NY 14203-1102 hwi.buffalo.edu<http://hwi.buffalo.edu/> Phone: (716) 898 8631 Fax: (716) 898 8660 Skype: eddie.snell Email: esn...@hwi.buffalo.edu<mailto:esn...@hwi.buffalo.edu> Webpage: https://hwi.buffalo.edu/scientist-directory/snell/ [hwilogo] Heisenberg was probably here! From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Tobias Beck Sent: Tuesday, June 9, 2020 9:46 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Highest resolution of X-ray / neutron / electron crystallography, cryo EM Dear all, Thanks for the link to the latest BioRxiv papers! So for cryo EM it is 1.2 now. Any numbers for neutron? Best, Tobias. Tobias Beck <tobiasb...@gmail.com<mailto:tobiasb...@gmail.com>> schrieb am Di. 9. Juni 2020 um 15:35: Dear all, I was asked by a student what the highest resolution is, for each of the four methods listed above. Maybe someone has researched the current numbers previously and would like to share them? For X-ray, I found 0.48 A in the PDB. For EM method details, the PDB gives me 0.6 A, but it is actually for electron diffraction. I found a structure with 1.8 A for Cryo EM. I am aware that resolution is only one parameter and that high resolution may not correspond to high data quality. However, maybe someone knows the record holders, either for biomacromolecules or small molecules or for both. Thanks! Best, Tobias. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
