You might get some clues from Acta Cryst. (2008). D64, 287-301 [ doi:10.1107/S0907444907067613 ] Glycerol concentrations required for the successful vitrification of cocktail conditions in a high-throughput crystallization screen R. Kempkes, et al.
and J. Appl. Cryst. (2002). 35, 538-545 [ doi:10.1107/S0021889802009238 ] The development and application of a method to quantify the quality of cryoprotectant solutions using standard area-detector X-ray images M. B. McFerrin and E. H. Snell both shamelessly building on the original work J. Appl. Cryst. (1996). 29, 584-587 [ doi:10.1107/S0021889896004190 ] Glycerol concentrations required for cryoprotection of 50 typical protein crystallization solutions E. F. Garman and E. P. Mitchell Most importantly check out J. Appl. Cryst. (1994). 27, 1070-1074 [ doi:10.1107/S0021889894008629 ] Flash freezing of protein crystals: investigation of mosaic spread and diffraction limit with variation of cryoprotectant concentration E. P. Mitchell and E. F. Garman as the cryocondition that causes a successful vitrification may need to be optimized further to get the best diffraction. Also - try room temp first so you know what your cryoprotectant is doing to your crystal. PS. Given the discussions room temperature crystals and the synchrotron, if you do this, make sure you keep the stream blowing at room temp. It still helps to remove heat that builds up. Cheers, Eddie ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Deepak Thankappan Nair [deepaktn...@gmail.com] Sent: Thursday, February 06, 2014 11:40 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] suggestions for cryoprotectant Hello, Does anybody know what would be a good cryoprotectant for the following condition: 800 mM Sodium phosphate monobasic/1200 mM Potassium phosphate dibasic 100 mM Sodium acetate/Aceticacid pH4.5 Thanks Deepak
