Re: [ccp4bb] enzyme or other protein with multiple cofactors

Hi Harry, can't resist to suggest 1PPR, peridinin-chlorophyll-protein from Amphidinium carterae, in which each polypeptide constitutes a scaffold with pseudo-twofold symmetry surrounding a hydrophobic cavity filled by two lipid, eight peridinin, and two chlorophyll a molecules. Best wishes, Ka

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Hi folks Another quick update - I am also parsing mmCIF (and also associated JSON) files from PDB - so if there are ligands in those files that I can identify unambiguously as being associated with *only one chain* (this is very important to me…), then I’ll include these as “ligands” in my stud

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Hi Harry, Maybe you could have a look at acetohydroxyacid synthase (AHAS). The catalytic subunit (dimer) contains FAD, ThDP and Mg2+ bound to the dimer interface. If you consider the holoenzyme complex (which consists of multiple catalytic and regulatory subunits) AHAS is bound to ATP too. There a

Re: [ccp4bb] enzyme or other protein with multiple cofactors

ggested?" >> – Dorothy Hodgkin >> From: CCP4 bulletin board on behalf of Harry Powell >> <193323b1e616-dmarc-requ...@jiscmail.ac.uk> >> Sent: 20 December 2024 10:37 >> To: CCP4BB@JISCMAIL.AC.UK >> Subject: [ccp4bb] enzyme or other protein with

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Hi, cytochrome P450 (heme + nadp) nitric oxide synthase (FAD, FMN, heme) ferredoxin-NADP+ reductase succinate dehydrogenase complex II (FASD + iron-sulfur) ribonucleotide reductase (Fe + Mn + tyrosyl radical) lactate oxidase (FMN + Ca) glutamate synthase dihydrooroate DH Lipoamid DH Xanthine oxida

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Hi folks Before anyone points this out, I notice from PDB 2BS2 (I’ve only looked on PDBe for this, RCSB and PDBj might differ in their “added value annotation"!) and UniProt P17596 that one calls iron-sulfur clusters “ligands” and the other calls them “cofactors”. For my current purposes, I’m

Re: [ccp4bb] enzyme or other protein with multiple cofactors

r 2024 10:37 *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* [ccp4bb] enzyme or other protein with multiple cofactors External Sender: Use caution. Hi folks I’m familiar with enzymes that have a single co-factor (for example haem in myoglobin [or in the individual chains in

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Glucokinase. Single chain protein binds to no of cofactors like ATP and others apart from it's substrate glucose. Thanks Zeya On Fri, 20 Dec, 2024, 2:48 pm Harry Powell, < 193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: > Wow. I was right about one thing - this _is_ a good place to ask. >

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Wow. I was right about one thing - this _is_ a good place to ask. Many thanks for the flood of responses - that will keep me busy for a while - I’ll get back to you with what I think are the most useful ones for my purposes (so many, many apologies to those people who have taken the time to resp

Re: [ccp4bb] enzyme or other protein with multiple cofactors

inada" De: CCP4 bulletin board em nome de Nichols, Charlie Enviado: sexta-feira, 20 de dezembro de 2024 11:17 Para: CCP4BB@JISCMAIL.AC.UK Assunto: Re: [ccp4bb] enzyme or other protein with multiple cofactors Hi Harry, PDB 1QL

Re: [ccp4bb] enzyme or other protein with multiple cofactors

For PSI, the reference models are 8BCV (plant) 2.2 Å with clashscore 5; and 7ZQC (algae) 2.3 Å, clashscore 3. Best, alexey > On 20 Dec 2024, at 19:08, Guillaume Gaullier > wrote: > > If you are going to use these atomic models, I highly recommend looking at > the maps (whether crystallograp

Re: [ccp4bb] enzyme or other protein with multiple cofactors

10:38 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] enzyme or other protein with multiple cofactors WARNING: This message was sent by an external party. Report suspicious messages via the “Report Phishing” button in Outlook. Hi folks I’m familiar with enzymes that have a single co-factor (for

Re: [ccp4bb] enzyme or other protein with multiple cofactors

ent: Friday, December 20, 2024 11:37:39 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] enzyme or other protein with multiple cofactors Hi folks I’m familiar with enzymes that have a single co-factor (for example haem in myoglobin [or in the individual chains in haemoglobins] or FAD in flavoprotein

Re: [ccp4bb] enzyme or other protein with multiple cofactors

ter if one could really 'see' whether molecules...were > just as experiments suggested?" > > – Dorothy Hodgkin > -- > *From:* CCP4 bulletin board on behalf of Harry > Powell <0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> > *Sent:* 20 Dec

Re: [ccp4bb] enzyme or other protein with multiple cofactors

'see' whether molecules...were just as experiments suggested?" – Dorothy Hodgkin From: CCP4 bulletin board on behalf of Harry Powell <193323b1e616-dmarc-requ...@jiscmail.ac.uk> Sent: 20 December 2024 10:37 To: CCP4BB@JISCMAIL.AC.UK Su