Hello Harry, I can think of an example of multiple protein chains with multiple cofactors: photosystems I and II. There are several structures in the PDB, take a look, they are full of chlorophylls, carotens, and other things. Definitely more than one per protein chain. If you are going to use these atomic models, I highly recommend looking at the maps (whether crystallographic or cryoEM), because some of the cofactors are strongly supported by the density while others much less so. Model building is difficult, no judgment here, just reporting an observation.
I can't think of an example of a single protein chain with multiple cofactors off the top of my head, but there probably are some out there. I hope this helps, Guillaume ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Harry Powell <0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> Sent: Friday, December 20, 2024 11:37:39 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] enzyme or other protein with multiple cofactors Hi folks I’m familiar with enzymes that have a single co-factor (for example haem in myoglobin [or in the individual chains in haemoglobins] or FAD in flavoproteins), but was wondering if there are examples of single-chain proteins that have multiple cofactors (or even multiple chain proteins that have multiple cofactors bound to a single chain)? I’m not bothered (at the moment) about proteins that have cofactors bound to different chains. I thought this would be a good place to ask… Harry ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ VARNING: Klicka inte på länkar och öppna inte bilagor om du inte känner igen avsändaren och vet att innehållet är säkert. CAUTION: Do not click on links or open attachments unless you recognise the sender and know the content is safe. När du har kontakt med oss på Uppsala universitet med e-post så innebär det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/ E-mailing Uppsala University means that we will process your personal data. For more information on how this is performed, please read here: http://www.uu.se/en/about-uu/data-protection-policy ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
