Hi Martin This brought back memories of my PhD when I was synthesising metallorganic molecules (quite, quite different from the species that are described nowadays as “metal-organic frameworks”) which had M-M distances in the range 2.8 - 3.1 Å). These species wouldn’t have survived a close encounter with a protein unless it had been dehydrated thoroughly and was under an argon atmosphere.
Best wishes Harry > On 20 Dec 2024, at 12:09, Martin Malý <martin.maly...@email.cz> wrote: > > Dear Harry, > > You can check this structure of an FAD-dependent monooxygenase with bound FAD > and NADPH: https://www.rcsb.org/structure/1k0j > However, authors of this review (https://doi.org/10.3390/ijms131215601) seem > not to be convinced about the NADPH position (if I remember that review > right). > > By the way, I would be interested in metal-metal interactions in biomolecules > (if I can a bit hijack this thread...) I do not mean interactions like > Mg-Cl-Mg, but clearly two metal atoms closer than ~3.6 A to each other. > > Best wishes, > Martin > > > On 20/12/2024 11:07, Chandra Prakash Tiwari wrote: >> Yes, multiple cofactors can exist in active sites. One example is Enolase. >> Also, metal-metal bonds are common in coordination spheres for catalysis. >> >> On Fri, Dec 20, 2024 at 4:34 PM David Briggs <david.bri...@crick.ac.uk> >> wrote: >> Hi Harry, >> >> The first thing that comes to mind is any sort of light harvesting complex - >> take a look at 1RWT. Would that fit the bill? >> >> D >> >> >> >> -- >> Dr David C. Briggs CSci MRSB (he/him) >> Principal Laboratory Research Scientist >> Signalling and Structural Biology Lab >> The Francis Crick Institute >> London, UK >> Working hours: Mon-Fri 0900-1700 >> == >> about.me/david_briggs | OrcID | Google Scholar >> == >> "Would it not be better if one could really 'see' whether molecules...were >> just as experiments suggested?" >> – Dorothy Hodgkin >> From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Harry Powell >> <0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> >> Sent: 20 December 2024 10:37 >> To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> >> Subject: [ccp4bb] enzyme or other protein with multiple cofactors >> >> >> External Sender: Use caution. >> >> >> Hi folks >> >> I’m familiar with enzymes that have a single co-factor (for example haem in >> myoglobin [or in the individual chains in haemoglobins] or FAD in >> flavoproteins), but was wondering if there are examples of single-chain >> proteins that have multiple cofactors (or even multiple chain proteins that >> have multiple cofactors bound to a single chain)? >> >> I’m not bothered (at the moment) about proteins that have cofactors bound to >> different chains. >> >> I thought this would be a good place to ask… >> >> Harry >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing >> list hosted by www.jiscmail.ac.uk, terms & conditions are available at >> https://www.jiscmail.ac.uk/policyandsecurity/ >> The Francis Crick Institute Limited is a registered charity in England and >> Wales no. 1140062 and a company registered in England and Wales no. >> 06885462, with its registered office at 1 Midland Road London NW1 1AT >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
