Hi folks,
I can not access to SSRL from yesterday. smbnxs1.slac.stanford.edu. or
smbnxs2.slac.stanford.edu
I wonder something is wrong in the server.
Please let me know what happened to the SSRL if you know that.
Thanks to all.
Daniel
experimental methods through time spent in the lab of Owen
Davies in Newcastle, this is a chance to work at the cutting edge of
applied bioinformatics.
thanks
Daniel
--
Prof Daniel Rigden
Institute of Integrative Biology
Room 101, Biosciences Building
University of Liverpool
Crown St., Liverpool, L69
ebsite is
January 21th 2020.
do not hesitate to circulate this announcement to potential candidates.
Best wishes
PS: do not contact me but register on the website
Daniel Levy
Team Molecular Microscopy of Membrane
UMR 168 Institut curie, France
https://science.institut-curie.org/team-levy
phone 33
scale protein production section
at the end.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/
Good luck with your search and finishing your PhD.
Best,
Dan
Daniel A Bonsor PhD.
Lu Lab
Institute of Human Virology
University of Maryland, Baltimore
725 W Lombard Street N370
Baltimore
Maryland
from
http://sourceforge.net/projects/mmview) that can be installed on the
user’s computer.
Petr Cech and Daniel Svozil
--
Daniel Svozil, PhD
Head of Laboratory of Informatics and Chemistry
Institute of Chemical Technology
Czech Republic
phone: +420 220 444 391
http://ich.vscht.cz/~svozil
http://www.cnio.es/ing/cursos/programadoctorado.asp or
contact our Training Office at p...@cnio.es.
Please, feel free to distribute this message on your local institute
network and thank you very much in advance for advertising our programmes
in your institution.
Best regards,
Daniel Lietha
Hello Gloria,
I usually let Coot display the nearest symmetry molecules (as CA) and save
those that fit: Option "Save Symmetry Coordinates..." and pick the
molecule to save.
Best regards, Daniel
--
Daniel Schlieper email: daniel.schlie...@tuxomania.net
Bi
You can find here several links concerning NMR
http://www.drorlist.com/nmr.html
with a discussion list:
https://listes.sc.univ-paris-diderot.fr/sympa/info/nmr
Daniel
Le 22/03/2012 19:35, Luthra,Amit a écrit :
Is any NMR blog available for discussion?
Amit Luthra, Ph.D.
Post-Doctoral
concentration of detergent during the early steps since the
lipid/detergent mixed micelles will be less denaturing than the pure
detergent micelles in the later steps. Thin layer chromatography is a
quick and easy method to check if you have a large amount of lipids in
your preparation.
HTH
Daniel
/programapostdoctorado-banco-santander.asp
Online application form:
https://www.cnio.es/ing/cursos/programapostdoctorado-form-uk.asp
Please, feel free to distribute this message on your local institute
network.
Best regards,
Daniel Lietha
Cell Signalling and Adhesion Group
Structural Biology and
Dear All,
I would like to inform you that an opening for a postdoctoral position is
available in the Structural Bases of Genome Integrity Group at the Spanish
National Cancer Research Centre in Madrid (CNIO, Madrid)
We seek for candidates with a PhD in structural biology, biochemistry or a
relate
"It refolds properly according to the CD spectra but it some how manages to
hold on to trace amounts of ligand despite serial dialysis (500ml to 5ml of
sample) in 8M, 6M, 4M, 2M 1M urea followed by 50mM Tris. I also have a homolog
that abjectly refuses to refold in either urea or guanidine, thou
The Fa" vector is always a 90 degree left turn from the Fa vector. For a
centrosymmetric heavy atom substructure such as 1 mercury site in P21,
the Fa" vector would point straight up or down.
hope that helps,
Citizen Dan
William Scott wrote:
Hi Citizens:
Try not to laugh.
I have an embar
Hi all,
This maybe a simple/stupid question. I collected data at the synchrotron,
integrated and scaled the data in P222 using HKL2000, though I should of scaled
the data as P212121.
When I try to reindex using Reindex I get a message of
You are changing the symmetry of merged data are
Have you looked at the Keio collection. Though they are not compatible with T7
promoters, you can use the λDE3 Lysogenization Kit to add the T7 polymerase.
A possible source is from Yale http://cgsc.biology.yale.edu/Auxotrophs.php
though of course there are others.
Dan
THERMATOGA MARITIMA IscU IS A STRUCTURED IRON-SULFUR CLUSTER ASSEMBLY PROTEIN
June 14, 2002 The Journal of Biological Chemistry, 277, 21397-21404.
His-tagged Iron cluster that runs as a doublet. Mass-spec show they are the
same species. They concluded "that the protein binds SDS in two stoichio
I am using the glutathione sepharose 4B beads from GE. I had noticed that the
capture efficiency does decrease with usage, though I have successfully
regenerated the beads at least 20 times and I am still using them.
I regenerate using 2CV of 6M guanidine after each run and every 5 runs with 70
AVER DOMAIN 1 should be followed by ROTA MATRIX and TRAN. What does "NCS
2 1" do? Is that in the documentation? I never saw that before, and I
don't see it now in blah/ccp4-6.1.3/doc/dm.doc . If you intend to refine
the NCS matrices, the instructions for that should be on the same line
with "AV
I have a His-tagged protein which I am coexpressing with it's binding partner
to prevent proteolysis. Once on the Nickel column I can remove 80% of the
partner by flushing 2l of 1.3M NaCl solution buffered at pH 8.5 overnight.
However the last 20% is difficult to remove, even if I reload the Nic
Maybe you should look at: "Rotation barriers in crystals from atomic
displacement parameters" Emily Maverick and Jack Dunitz (1987) Vol
62(2), 451-459.
They reported that the libration amplitude of one part of a crystalline
small molecule relative to another part is strongly correlated to bond
The journal was Molecular Physics (1987) Vol 62(2), 451-459.
(my brain librates when I type)
Daniel Anderson wrote:
Maybe you should look at: "Rotation barriers in crystals from atomic
displacement parameters" Emily Maverick and Jack Dunitz (1987) Vol
62(2), 451-459.
They reporte
I wish to thank everyone. I did try shifting to a higher pH and flushing 3l of
salt solution over the column which did not work. I tried 20ml 2M Urea on the
column and a stepwise shift to no urea that showed removal of the protein. I
will try binding studies to see if I did not denature the His
Producing the proteins in cell free system or bacterial expression can affect
the removal of the start methionine. Although the same rules of a small amino
acids next to the start methionine apply, different methionine aminopeptidases
tolerate certain small ones better. Depending on the which ce
Hydroxyapatite can be miraculous, but is often very capricious: among
others, it is temperature sensitive and two different corners of a
somewhat inhomogeneous cold room may provide different results.
Daniel
Le 29/11/2010 16:08, Sebastiano Pasqualato a écrit :
Hi all,
I read/heard that
to any references that show a
different pH sensing mechanism (other than His)? Thanks.
Best,
Daniel
I would like to point out that HSQC could still be applied even in such a large
protein. TROSY-HSQC has been successful in improving peaks in spectra of large
protein. Typically the sample would need to be deuterated to see the full
effect of TROSY, but even a partial deuteration can improve sig
On behalf of Mar Pérez (Head of CNIO Training Office)
Dear colleague,
We would like to draw your attention to the calls for the International
Ph.D. and Postdoctoral Programmes at the Spanish National Cancer Research
Centre (CNIO) in Madrid. We would greatly appreciate if you could forward
the
work, and is equipped with
a state of the art facilities. The Grenoble laboratory is located on an
international research campus providing one of the best environments for
structural biology in Europe.
Further details are available here:
http://www.embl.de/training/postdocs/eipod/index.html
--
D
We have used Alphalyse (http://www.alphalyse.com/picknpost.html).
Dan
In the Wyatt light scatter training class, they told us to set the
alignment parameters ONLY with a monodisperse peak, such as the monomer
peak from "monomeric BSA", formerly Sigma Chemical A-1900. The BSA
alignment parameters are then manually typed as inputs for the
scientifically interesting
If you want a ClpP minus strain you can get it from the Keio strains.
http://cgsc.biology.yale.edu/index.php
http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp
For expression with the T7 promotor, you will need to use the λDE3
Lysogenization Kit or use a Arabinose induction plasmid instead.
Factor Xa will work depending on the exact sequence. If your sequence starts
MRS... and the start methionine is important and not removed then yes it will
work when you clone it into a Factor Xa site. If however the start sequence
starts RS... and the start methionine is actually removed, when y
/cursos/descargas/doctorado/Poster_PhD_2011.pdf
For further information, please contact our Training Manager, Mar Pérez at
p...@cnio.es.
Thank you,
Daniel Lietha
Cell Signalling and Adhesion Group
Structural Biology and Biocomputing Programme
Spanish National Cancer Research Centre (CNIO)
C
PMID: 20508091.
with the cited literature
Daniel
Le 04/03/2011 17:31, Justin Hall a écrit :
Dear Community,
In trying to trouble shoot an experiment I have become interested in the
cellular process that regulates the insertion and proper orientation of
membrane proteins. I am looking for
If the protein is His-tagged, load the purified protein on to a Nickel column
and place the column in a water bath above the protein's melting temperature.
Recycle 5mls of water through the column to collect the peptide and then dry
freeze or speed vac the sample to concentrate the peptide.
I
Is the PISA server having problems? I can not seem to upload any structure.
Thanks
Dan
It has eventually loaded but has taken nearly 20minutes.
Thanks for all the replies. Like I said, it eventually did upload the structure
after 20 minutes and I had no problems in analyzing the interface. I did try
two different computers, my home computer on Friday night and the lab one on
Saturday afternoon. At home it failed to load the structure, b
Or try SCWRL from Dunbrack's lab...
--- On Thu, 5/12/11, gauri misra wrote:
From: gauri misra
Subject: Re: [ccp4bb] mutation and minimization
To: CCP4BB@JISCMAIL.AC.UK
Date: Thursday, May 12, 2011, 12:52 PM
You can try Swiss-PDB viewer..
2011/5/12 Thomas Holder
Hi Andreas,
I would like
ply online. Informal
enquiries can be made to Daniel Kloer (daniel.kl...@syngenta.com).
Protein Scientist
£28,000 - £35,000 + benefits
Jealott’s Hill, Bracknell, Berkshire
As an independent worker, you will be a part of our Protein Production
Team, providing protein expression/purification support in a timely
manner across a range of Crop Protection Research projects. This will
invol
).
EMBL is seeking to recruit postdoctoral researchers to join the group of
Daniel Panne at EMBL Grenoble. The Panne group studies molecular mechanisms of
epigenetic gene regulation and signaling in the innate immune system. We are
seeking to recruit outstanding candidates with a strong interest in
Jalview is another option.
http://www.jalview.org/
Sent from my HTC
- Reply message -
From: "Yuri"
Date: Fri, Aug 19, 2011 00:31
Subject: [ccp4bb] Sequence Alignment Question
To:
UID 23435 BODY[1]<0> {238}
Hello Everyone,
A little off topic but, what is a good way to show (publicatio
ined in terms of its
depth. However, I think maintaining a focus on the fundamentals [...] will
provide a useful foundation for those who want to apply the skills on their
own."
"Gaël McGill gave a brilliant [molecular animation] presentation and tutorial".
Please feel free to e-m
Hi Jane,
the Whatif server lists the secondary structure according to DSSP.
http://swift.cmbi.ru.nl/servers/html/index.html
-> Protein analysis -> Secondary Structure, symmetry and accessibility
Best regards, Daniel
--
Daniel Schlieper email: daniel.schlie...@tuxoman
Europe’s cultural capitals
Applications with CV and inquiries should be sent by email to the group
leader, Dr. Daniel Lietha (dlie...@cnio.es). All enquiries and applications
will be treated confidentially.
A cheaper solution than buying the dialysis buttons would be just to make your
own out of the top of a microtube. Depending on what volume you want to dialyse
you can use either a PCR tube (~35 uL per lid) or larger 1.5 mL tube (~250 uL).
Just cut the top off the tube using a hot scalpel, you th
How do I convert the B-factors from my final structure to full B factors if I
did not use TLS refinement? I have been refining isotropic B-factors. Do I
switch to overall B-factor refinement, do something else, or have I missed the
point altogether?
It may be a dumb question but best to be saf
Hello again...
I have a 2.7A resolution data set. Spacegroup P212121 as suggested by Pointless
and best space group when run through Phaser. Unit cell is
110.02x160.49x186.55. Mathew's suggest 11 complexes in ASU. Both Molrep and
Phaser can only find 6. This seems to be a typical observation wi
analyse the
success of MR using PHASER or other software. You will participate in
manuscript preparation.
Full details and instructions on how to apply are here
http://www.liv.ac.uk/working/job_vacancies/research/R-572008.htm
Informal enquiries may be made to me.
--
Dr Daniel John Rigden
Hello again!
Following my previous question, there was something wrong with the staring
model for molecular replacement. Now that is sorted, I have 8 complexes in the
ASU. After a few rounds of refinement with NCS and isotropic Bfactors, both the
Rfactor and Rfree get stuck at 30% and 36%, resp
(What is in the Hollywood district other than tourists looking for
"Hollywood"?)
I have a silly anecdote that does not answer Harry's question:
It must have been 1995 or 1996, the Beckman sales representative
appeared at my desk on a Friday afternoon without an appointment, but
carrying a bri
Hello again.
At first I was not worry but maybe now I am. I have completed a structure and
submitted to the PDB. They queried my Rsym value in the highest resolution bin,
2.5-2.37A (may I dare say it 100%). I was not worried at the time as I had:
99.4% completeness
Mean(I/sdI) of 2.5
and a redu
biophysical instrumentation is provided. To apply please send your CV, a
statement of research interests, and names (including email address) of at
least two referees by email to Dr. Daniel Panne (pa...@embl.fr). For more
information: http://www.embl.fr/research/unit/panne/index.html
and integriry of the protein.
HTH
Daniel
Le 13/07/2010 00:59, Pascal Egea a écrit :
Dear All,
I apologize for the not strictly crystallography-related query.
I am currently purifying several membrane proteins solubilized in
fos-cholines detergents and I consistently observe a significant loss of
Does anyone know if the ZM-M215W and ZM-M240W Zalman monitors work in 3D
with Coot and Pymol (would use them on Mac OS10.6)? If so, does the
increased resolution improve things compared to the ZM-M220W?
Thanks,
Daniel
Refinement with rigid-base TLS parameterization has been previously
explored:
Holbrook, Dickerson, Kim (1985) Acta Cryst B41, 255-262.
(the photocopy is located in the pile of dust that I maintain adjacent
to my desk)
Ethan Merritt wrote:
On Thursday 15 July 2010, Huw Jenkins wrote:
Hi,
and treat it
as a soluble protein? Or something else?
Thanks in advance for all of your suggestions.
Daniel Bonsor
It behaves very well. No precipitation/cloudiness during purification.
Secondary structure estimation by CD is ~50% alpha helical, 25% beta-sheet and
25% random coiled. I screened at 2, 4, 6 and 8mg/ml (8mg/ml around about
60:40% precipitation). If any other information is needed, just ask.
Th
By size exclusion, it eluted where a 30kDa protein would be expected to elute.
As for functional assay, I am currently trying to express its binding partner,
though it shows a lot of degradation. But when I mix both proteins and run by
size exclusion it causes the peaks of its binding partner to
Another very nice script to run and collect statistics from XDS is
xdsme from Pierre Legrand
http://code.google.com/p/xdsme/
Daniel
Le 05/08/2010 16:55, Jovine Luca a écrit :
WRT the redundancy, I am afraid you have to recompute an approximate value
yourself using the number of
).
Crystal size is more than 0.4 um.
I tried to do annealing, dehydration and glutaraldehyde use but It
does not increase the diffraction.
I need some help.
I will very appreciate your all comments.
Thanks
Best,
Daniel
crystallographic data.
Daniel
Le 14/09/2010 02:31, xingliang zhang a écrit :
Dear everyone,
Recently，we collected data of a native protein crystal on synchrotron
in Shanghai. When we did with the original data with HKL2000, we found
an unconversant phenomenon ,just as the picture in the enclosure, there
An old one:
Eichele G, Ford GC, Jansonius JN. Crystallization of pig mitochondrial
aspartate aminotransferase by seeding with crystals of the chicken
mitochondrial
isoenzyme. J Mol Biol. 1979 Dec 5;135(2):513-6. PubMed PMID: 537086.
Daniel
Le 21/09/2010 22:24, Christopher Rife a écrit :
Hi
There is a nice paper
Comput Biol Chem. 2009 Dec;33(6):445-50. Epub 2009 Oct 20.
Predicting melting temperature directly from protein sequences.
Ku T, Lu P, Chan C, Wang T, Lai S, Lyu P, Hsiao N.
They have a list of 35 different proteins with their Tms with the references
from where they obtain
According to Pierce TCEP is "more" tolerant of nickel and cobalt. However, TCEP
is inactivated by other metals, namely copper, magnesium, silver and zinc.
Dan
Daniel A. Bonsor,
Boston Biomedical Research Institute,
64 Grove Street,
Watertown,
MA 02472 USA
crystallization can generate a mixture of different species with one of the
fragments being more readily crystallizable. There is only one way to find
out...
Daniel A. Bonsor,
Boston Biomedical Research Institute,
64 Grove Street,
Watertown,
MA 02472 USA
There are a couple of papers...
Acta Cryst. (2010). F66, 346-351
Crystallization and X-ray diffraction studies of cellobiose phosphorylase from
Cellulomonas uda
The space group was originally P21. During collection the crystal moved out of
the beam (and possibly the cyrostream). Upon recenter
):313-27.
PubMed PMID: 6302273.
Daniel
Le 30/09/2010 16:56, Daniel Bonsor a écrit :
There are a couple of papers...
Acta Cryst. (2010). F66, 346-351
Crystallization and X-ray diffraction studies of cellobiose phosphorylase from
Cellulomonas uda
The space group was originally P21. During
Is there something I am doing wrong in input file or I should fix these
parameters ? If yes how ?
Thanks in advance
Cheers
Daniel
An IUCr nomenclature committee once recommended eradication of the
"B-factor". Trueblood, et al. Acta Cryst (1996) A52, 770-781.
Whether or not the factor of 8 pi squared can be eradicated,
the publication is worth reading because it discusses what is modeled
by Ueq (times 8 pi squared = B), and it
Are you sure that you want AKTA anything? They are so expensive.
We have had good service from the old style ("HR") Bio-Rad Bio-Logic
chromatography systems. The new ones are called "DuoFlow".
-Dan
On Tue, 13 Feb 2007, Frank Lee wrote:
>
> Dear all,
>
> I need to decide between buying an AKTA p
My direct experience is with the old "HR" pumps, but my remarks apply to
the newer "DuoFlow".
On Day Zero, I disassemble the pump heads in the order specified in the
manual. I re-assemble with grease on the threads, thus preventing
expensive repairs due to corrosion of the bolts caused by salty bu
That was cryptic.
My point was that the BioRad pumps are very reliable given some basic
care. Its users quickly learn how to use it, as mentioned previously.
On Wed, 14 Feb 2007, Daniel Anderson wrote:
> My direct experience is with the old "HR" pumps, but my remarks apply t
It still seems to me strange that Bio-Rad would build a machine that can
reach pressures suitable for reverse-phase chromatography, but then not
include software control for gradual pump acceleration. I tried to
communicate to BioRad corporate suits that many HPLC columns are too
fragile for abrupt
this combination of NCS together in DM or
resolve ?
Is there something I am missing ?
Thanks in advance
Daniel
-- Forwarded message --
From: Daniel Adams <[EMAIL PROTECTED]>
Date: Feb 16, 2007 12:29 PM
Subject: Re: [ccp4bb] pseudotranslation and dyads in NCS
To: [EMAIL PROTECTED]
My apology for not mentioning unit cell,
The P1 unit cell is 74 91 116 109 105 90
Any suggesti
You may have called the version of phaser installed in the phenix path. Type
wich phaser
in a terminal to check it.
Daniel
yang li a écrit :
Hi:
I am using CCP4 6.0.2, it has phaser contained in this package. When
I use it
to do mr, error occured
good solution (introduction of selmet makes the protein ustable)
to make heavy metal derivatives?
Best Regards
Daniel
gards, Daniel
--
Daniel Schlieper email: [EMAIL PROTECTED]
Molecular Motors Group phone: +44 1883 722306 (x 305)
Marie Curie Research Institute fax : +44 1883 714375
The Chart, Oxted RH8 0TL, UK web : http://mc11.mcri.ac.uk
VACANCY NOTICE
Laboratoire Européen de Biologie Moléculaire
European Molecular Biology Laboratory
POSTDOCTORAL FELLOWSHIP IN STRUCTURAL BIOLOGY
Duty Station:Grenoble, France
Commencing Date: As soon as possible after closing date
Job Description:
Highly motivated postdoctoral candidates are
Hi Jacob,
as far as I understand it, proteins also oligomerise to share a stable
core. For example, some proteins form tetramers and minimise the material
needed to form a scaffold around their active centres. Could that be the
case for your protein as well?
Best regards, Daniel
--
Daniel
Hi,
I am working on a 60 kDa C. elegans protein that is predicted to be mostly
alpha-helix. It is over-expressed in E.coli and the yield is about 1 mg/L of
cell culture. The CD spec at 4 degree showed the presence of dominant
alpha-helix. However, we dont have any functional assay to conf
detergent. I stop here because I find
always very difficult to extrapolate the effect of detergent from
membrane protein to soluble protein.
Daniel
Daniel Jin a écrit :
> Hi,
>
> I am working on a 60 kDa C. elegans protein that is predicted to be
> mostly alpha-helix. It is over-express
this value calculated at each refinement step?
Best regards, Daniel
On Mon, 1 Oct 2007, Ian Tickle wrote:
> [...]
> we are working with an observation/parameter count ratio of say <
> 3 (naturally I'm counting the geometric restraints with the X-ray
> observations).
>
s renamed map
(e.g. fwt.ccp4) can be opened in pymol. [...]"
--
Daniel Schlieper email: [EMAIL PROTECTED]
Molecular Motors Group phone: +44 1883 722306 (x 305)
Marie Curie Research Institute fax : +44 1883 714375
The Chart, Oxted RH8 0TL, UK web
His6. Many other structure of membrane protein have used His-tag, but
still not enough to make sound statistics.
Daniel
deliang a écrit :
Hi there,
I purified a membrane protein with traditional His-tag on the C
terminal. Before crystallization, I wonder how this tag may affect the
result
Hi,
I am wondering whether there is a way to turn a membrane protein with known
crystal structure into a water soluble protein by systematic mutagenesis. I
guess it should be doable if we introduce enough hydrophilic residues on the
surface. Has anyone tested this crazy idea before? Thank
Hi,
I have been trying to express a rat protein in bacteria. The MBP-fusion
expressed at very high level (~ 40 mg/L) while the GST-fusion and His-tag only
gave inclusion bodies. The problem is that all protein runs in the void volume
on a size-exclusion column (s-200, hepes, pH 7.4, 200 mM
Hi,
I have a protein with two independently folded domains. I can express either
one in bacteria with pretty good expression yield. However, when I put them
together with a linker, the expression drops significantly. I can barely see
any soluble protein and most of it is now inclusion bodi
Jan,
Crystals containing haem protein are not necessarily red, the colour
depends on the oxidation state of the haem and on the haem/protein
ratio. A FeIII haem will look yellowish, gold or brown depending on
the haem/protein ratio, while an FeII haem will look more pink or red.
Daniel
Ian
Hi,
has anybody tried 3dconnexion's space mouse with O, coot or ccp4mg?
How does it compare to a normal mouse or to a 6 knob rotary encoder?
http://www.3dconnexion.com/
Best regards, Daniel
--
Daniel Schlieper email: [EMAIL PROTECTED]
Molecular Motors
COOT and CCP4mg ?
--
***
Daniel Anderson, Ph.D.
Email: [EMAIL PROTECTED]
Phone: 310-206-3642 Fax: 310-206-3914
Howard Hughes Medical Institute at
University of California Los Angeles
Lab: Paul Boyer Hall Room 219
For US Postal Service and 2-dimensional, use:
Box 951662 MRL5-748
Los Angeles, CA 90095-1662
Posted on behalf of Jean-Luc Popot; please send enquiries/applications
to him directly ([EMAIL PROTECTED]) :
Position offer
Physico-Chimie Moléculaire des Membranes Biologiques,
CNRS/Université Paris 7 UMR 7099,
IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris – France
Post-doctoral position
Hi there,
Sorry for the off topic questions. We need your feedback.
We are expressing a rat protein in insect cells. It is expressed as a secreted
protein with an N-terminal 6xHis tag. We can get about 4 mg of it from 1L
culture and everything looked quite normal at the very beginning (at 4C)
after the
'planarity-restraints' statement.
best,
Daniel
On Jul 8, 2008, at 1:00 AM, CCP4BB automatic digest system wrote:
does anyone know how to change the weighting of the Watson-Crick base
pair restraints using the dna-rna_restraints.def restraints definition
file in CNS? We are
.
Best regards, Daniel
--
Daniel Schlieper email: [EMAIL PROTECTED]
Molecular Motors Group phone: +44 1883 722306 (x 305)
Marie Curie Research Institute fax : +44 1883 714375
The Chart, Oxted RH8 0TL, UK web : http://mc11.mcri.ac.uk
On Fri, 18 Jul
Dear Jinjin Zhang,
what happens if you treat your data as perfect twin? Maybe you do have a
twin after all.
Best regards, Daniel
--
Daniel Schlieper email: [EMAIL PROTECTED]
Molecular Motors Group phone: +44 1883 722306 (x 305)
Marie Curie Research Institute
Hi,
We would like to order two peptides for co-crystallizaiton. I notice that there
is a big price jump for peptide over 95% pure and over 98% pure. Do you think
95% purity is good enough? Another choice is to order crude peptides and purify
by ourselves. Crude peptide is much cheaper. But we
ition of ClustalW did not
oppose a problem. Can anybody help us? Where do we have to put Fasta35 in
order to continue the installation of MrBump? Or is something possibly wrong
with the permissions (global or local)?
If anyone has an idea, please let us know!
Thanks a lot in advance!
Daniel
D
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