Hi bb, I am working on P1 dataset, SAD phasing resolution is 3.5 and trying to build model. However I am facing difficulties in improving map using NCS (in DM and resolve ). Mathews content and SRF indicate that there are 8 molecules and most likely two tetramer is ASU. There is also pseudotranslation (orthogonal vector is:11.7 0 0) Biochemically it shows that there is a dimer as basic unit i.e. two dimer in one tetramer.
Using Se HA site, findncs indicates following 2 folds (in polar angle): 1. 23.5 162 180, translation_orthogonal: 0.14 22.7 -3.13 2. 9.2 88.14 180,translation_orthogonal: -71.5 -0.25 0.41 3. 95.4 180 180, translation_orthogonal: -0.07 -68.1 -2.67 4. 90 179 180, trnaslation_orthogonal: 0.29 22.6 -2.8 5. 90 1.3 180, translation_orthogonal: -0.5 22.7 -2.8 However when I use above 2 folds as NCS, map doesn't improve at all... My struggle is how can I use this combination of NCS together in DM or resolve ? Is there something I am missing ? Thanks in advance Daniel
