In the Wyatt light scatter training class, they told us to set the
alignment parameters ONLY with a monodisperse peak, such as the monomer
peak from "monomeric BSA", formerly Sigma Chemical A-1900. The BSA
alignment parameters are then manually typed as inputs for the
scientifically interesting data. These alignment parameters only change
when the tubing is replaced.
I connected the UV and (old-style Wyatt) refractometer analog cables to
the miniDAWN, which contains an analog-to-digital converter. The
detector signals should stay synchronized in the digital data stream to
the computer.
My recollection is that on only one occasion, the data stream from the
miniDAWN went out of synchronization with the computer, so the software
recorded random garbage. I cured the problem via re-boot. The caveat is
that this was the first Windows NT computer that Wyatt's software had
ever seen. I loaded this specific Windows NT computer into the back of
my car and took it with me on a visit to Santa Barbara where I delivered
it to Wyatt and they built their software onto this specific computer.
There may be a latent bug in this specific Wyatt installation.
Which should make me feel special and unique.
-Dan
tommi kajander wrote:
Hi,
if you happen to have Wyatt's light scattering detector + their RI
detector rEX, together with analytical HPLC/SEC system
what are your experiences on the stability of the aligment? the
digital connections dont seem to work for us. ie. apparently
synchronizing the signals from different detectors (computers) to the
analysis software computer dont work, even if the alignment
(ie delay volumes between detectors) has been done.
Should work with analog cables to one detector and then LAN connection
from there on to the analysis PC. testing it.quit certain it will.
but i would be happy to hear if anyone had similar problems....
Thanks!
tommi
Tommi Kajander, Ph.D., Docent
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
