---------- Forwarded message ---------- From: Daniel Adams <[EMAIL PROTECTED]> Date: Feb 16, 2007 12:29 PM Subject: Re: [ccp4bb] pseudotranslation and dyads in NCS To: [EMAIL PROTECTED]
My apology for not mentioning unit cell, The P1 unit cell is 74 91 116 109 105 90 Any suggestions or comments will be appreciated... Best regards Daniel On 2/16/07, Eleanor Dodson <[EMAIL PROTECTED]> wrote:
To understand this one at least needs the P1 unit cell.. Operators 3 4 and 5 are almost the same rotation.. It is unlikely that they are all 2 folds? Eleanor Daniel Adams wrote: > Hi bb, > I am working on P1 dataset, SAD phasing resolution is 3.5 and trying > to build model. However I am facing difficulties in improving map > using NCS (in DM and resolve ). > Mathews content and SRF indicate that there are 8 molecules and most > likely two tetramer is ASU. There is also pseudotranslation > (orthogonal vector is: 11.7 0 0) > Biochemically it shows that there is a dimer as basic unit i.e. two > dimer in one tetramer. > > > > Using Se HA site, findncs indicates following 2 folds (in polar angle): > > 1. 23.5 162 180, translation_orthogonal: 0.14 22.7 -3.13 > 2. 9.2 88.14 180,translation_orthogonal: -71.5 -0.25 0.41 > 3. 95.4 180 180, translation_orthogonal: -0.07 -68.1 -2.67 > 4. 90 179 180, trnaslation_orthogonal: 0.29 22.6 -2.8 > 5. 90 1.3 180, translation_orthogonal: - 0.5 22.7 -2.8 > > However when I use above 2 folds as NCS, map doesn't improve at all... > My struggle is how can I use this combination of NCS together in DM or > resolve ? > Is there something I am missing ? > > > Thanks in advance > > > Daniel >
