Hydroxyapatite can be miraculous, but is often very capricious: among others, it is temperature sensitive and two different corners of a somewhat inhomogeneous cold room may provide different results.
Daniel

Le 29/11/2010 16:08, Sebastiano Pasqualato a écrit :
Hi all,
I read/heard that hydroxyapatite column can be used to purify proteins, getting 
separation results orthogonal to ion exchange and size exclusion chromatography.
I was wondering if any of you would be kind enough to share her/his experience 
with me, and would suggest vendors and models for such columns.
Thanks in advance,
best,
s

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