Re: [ccp4bb] Crystallizing a membrane associated protein

[Daniel Bonsor]

By size exclusion, it eluted where a 30kDa protein would be expected to elute. 
As for functional assay, I am currently trying to express its binding partner, 
though it shows a lot of degradation. But when I mix both proteins and run by 
size exclusion it causes the peaks of its binding partner to elute earlier 
suggesting that they are interacting.