Hi, What size are these crystals? Chances are, you could take them as they
are (with cryo-protection and cryo-cooling, of course) to a synchrotron
beamline with a small beam.
Also, what is the PI of the protein? If crystals grow at both pH 5.5 and
8.5, perhaps you could set up a grid with pH in one
On 05/12/2024 17:30, Vitali James wrote:
I am visualizing the map in coot and chimera. How to view the map at
the same unit or at the same contour in coot and chimera. For example
if a map viewed in coot at 2 rmsd, how to adjust or convert the same
contour to chimera map level. As chimerax u
Thank you very much for your reply to my question on how to optimize
microcrystal. In response to your suggestions and questions, I would like to
make the following reply:
First of all, my protein can grow crystals like this one in a variety of
situations.
For example, other conditions:
1.15% PE
Hi,
Are these initial crystals or have you already attempted some optimization?
>From the crystallization condition, it appears these are your first
crystals. If so, you could try the following:
1) screen around this condition for each component separately or together
(coarse grid and fine scree
If you haven't already, you should definitely try MMS microseeding (adding
seed stock to a random screen). There’s a good chance you might be able to
get new hits in different conditions.
More info: https://www.douglas.co.uk/mms.htm
Best regards,
Stefan
*Stefan Kolek*
Douglas Instruments Ltd
D
Mapmask with:
SCALE SIGMA
Would also normalise the map.
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
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Original Message
On 05/12/2024 17:58, Foster, Samuel wrote:
> In chimerax the commands for altering maps is described in
> https://www
Hi,
I am visualizing the map in coot and chimera. How to view the map at the
same unit or at the same contour in coot and chimera. For example if a map
viewed in coot at 2 rmsd, how to adjust or convert the same contour to
chimera map level. As chimerax uses the absolute density value for maps
no
In chimerax the commands for altering maps is described in
https://www.cgl.ucsf.edu/chimerax/docs/user/commands/volume.html#level with
rmsLevel and sdLevel giving coot like results,
The volume viewer panel also gives an interactive element if you'd prefer?
Bw,
Samuel Foster MBioChem / Molecul
Hello, I agree that it looks difficult. I would try lowering the protein
concentration with that condition. Seeding springs to mind. What is in the
purification buffer?
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
Sent from Proton Mail Android
Original Message
On 05/1
Hello, I would recommend a look at:
gemmi sf2map --normalise
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
Sent from Proton Mail Android
Original Message
On 05/12/2024 17:30, Vitali James wrote:
> Hi,
> I am visualizing the map in coot and chimera. How to view the map
20% PEG 1500; 0.2M MgCl2;0.1M Tris pH 8.5
I would like to ask how to optimize such crystals?
We have no similar situation, crystal composition as shown
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