If you haven't already, you should definitely try MMS microseeding (adding seed stock to a random screen). There’s a good chance you might be able to get new hits in different conditions.
More info: https://www.douglas.co.uk/mms.htm Best regards, Stefan *Stefan Kolek* Douglas Instruments Ltd Douglas House, East Garston, Berkshire, RG17 7HD, UK ste...@douglas.co.uk Tel: +44 1488 649090 US toll-free: +1-877-225-2034 www.douglas.co.uk On Thu, 5 Dec 2024 at 17:52, Jon Cooper < 0000488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote: > Hello, I agree that it looks difficult. I would try lowering the protein > concentration with that condition. Seeding springs to mind. What is in the > purification buffer? > > Best wishes, Jon Cooper. > jon.b.coo...@protonmail.com > > Sent from Proton Mail Android > > > -------- Original Message -------- > On 05/12/2024 15:03, 白雪慧 wrote: > > 20% PEG 1500; 0.2M MgCl2; 0.1M Tris pH 8.5 > > I would like to ask how to optimize such crystals? > We have no similar situation, crystal composition as shown > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
