If you haven't already, you should definitely try MMS microseeding (adding
seed stock to a random screen). There’s a good chance you might be able to
get new hits in different conditions.

More info: https://www.douglas.co.uk/mms.htm

Best regards,

Stefan


*Stefan Kolek*
Douglas Instruments Ltd
Douglas House, East Garston, Berkshire, RG17 7HD, UK
ste...@douglas.co.uk
Tel: +44 1488 649090
US toll-free: +1-877-225-2034
www.douglas.co.uk


On Thu, 5 Dec 2024 at 17:52, Jon Cooper <
0000488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote:

> Hello, I agree that it looks difficult. I would try lowering the protein
> concentration with that condition. Seeding springs to mind. What is in the
> purification buffer?
>
> Best wishes, Jon Cooper.
> jon.b.coo...@protonmail.com
>
> Sent from Proton Mail Android
>
>
> -------- Original Message --------
> On 05/12/2024 15:03, 白雪慧 wrote:
>
> 20% PEG 1500; 0.2M MgCl2; 0.1M Tris pH 8.5
>
> I would like to ask how to optimize such crystals?
> We have no similar situation, crystal composition as shown
>
>
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