Thank you very much for your reply to my question on how to optimize microcrystal. In response to your suggestions and questions, I would like to make the following reply: First of all, my protein can grow crystals like this one in a variety of situations. For example, other conditions: 1.15% PEG 8000;0.2M MgCl2;0.1M HEPES pH 7.5 2.20% PEG 1500;0.2M MgCl2;0.1M HEPES pH 7.5 3.25% PEG 8000;0.2M NaCl;0.1M NaCaco pH 5.5 4.20% PEG 1e500;0.2M MgCl2;0.1M Tris pH 8.5 Second, my crystals grow within three days, and there is no phosphate in the protein solution. Finally, in fact, I tried crystal inoculation before, but did not find any change in crystal shape. Maybe my operation was also wrong. I sucked out the crystal, mashed it, diluted it in series, and removed the primary crystal condition, and the protein concentration did not change, nor did the concentration of the pool liquid change greatly
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