Hello, I agree that it looks difficult. I would try lowering the protein concentration with that condition. Seeding springs to mind. What is in the purification buffer?
Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent from Proton Mail Android -------- Original Message -------- On 05/12/2024 15:03, 白雪慧 wrote: > 20% PEG 1500; 0.2M MgCl2;0.1M Tris pH 8.5 > > I would like to ask how to optimize such crystals? > We have no similar situation, crystal composition as shown > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
