Hi, Are these initial crystals or have you already attempted some optimization?
>From the crystallization condition, it appears these are your first crystals. If so, you could try the following: 1) screen around this condition for each component separately or together (coarse grid and fine screen) 2) try lower protein concentration and variation of crystallization temperature 3) micro or macro seeding 4) how large are the crystals? can try open the drop, harvesting a few of the better/larger ones that are single (poke around to separate) and try X-ray diffraction screening (including a micro focus beam) to get some initial characterization of diffraction, and if lucky even get a unit cell/space group to estimate/validate crystal components/composition, verify protein crystals, and if very lucky, may even get an initial data set and/or preliminary structure. Regards, Debanu — Debanu Das On Thu, Dec 5, 2024 at 23:04 白雪慧 <zb20193020...@cau.edu.cn> wrote: > 20% PEG 1500; 0.2M MgCl2; 0.1M Tris pH 8.5 > > I would like to ask how to optimize such crystals? > We have no similar situation, crystal composition as shown > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
