Hi, What size are these crystals? Chances are, you could take them as they
are (with cryo-protection and cryo-cooling, of course) to a synchrotron
beamline with a small beam.
Also, what is the PI of the protein? If crystals grow at both pH 5.5 and
8.5, perhaps you could set up a grid with pH in one direction and protein
concentration in the other?
Good luck!
Nukri

On Fri, Dec 6, 2024, 10:18 白雪慧 <zb20193020...@cau.edu.cn> wrote:

> Thank you very much for your reply to my question on how to optimize
> microcrystal. In response to your suggestions and questions, I would like
> to make the following reply:
> First of all, my protein can grow crystals like this one in a variety of
> situations.
> For example, other conditions:
> 1.15% PEG 8000;0.2M MgCl2;0.1M HEPES pH 7.5
> 2.20% PEG 1500;0.2M MgCl2;0.1M HEPES pH 7.5
> 3.25% PEG 8000;0.2M NaCl;0.1M NaCaco pH 5.5
> 4.20% PEG 1e500;0.2M MgCl2;0.1M Tris pH 8.5
> Second, my crystals grow within three days, and there is no phosphate in
> the protein solution.
> Finally, in fact, I tried crystal inoculation before, but did not find any
> change in crystal shape. Maybe my operation was also wrong. I sucked out
> the crystal, mashed it, diluted it in series, and removed the primary
> crystal condition, and the protein concentration did not change, nor did
> the concentration of the pool liquid change greatly
>
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