Hi, What size are these crystals? Chances are, you could take them as they are (with cryo-protection and cryo-cooling, of course) to a synchrotron beamline with a small beam. Also, what is the PI of the protein? If crystals grow at both pH 5.5 and 8.5, perhaps you could set up a grid with pH in one direction and protein concentration in the other? Good luck! Nukri
On Fri, Dec 6, 2024, 10:18 白雪慧 <zb20193020...@cau.edu.cn> wrote: > Thank you very much for your reply to my question on how to optimize > microcrystal. In response to your suggestions and questions, I would like > to make the following reply: > First of all, my protein can grow crystals like this one in a variety of > situations. > For example, other conditions: > 1.15% PEG 8000;0.2M MgCl2;0.1M HEPES pH 7.5 > 2.20% PEG 1500;0.2M MgCl2;0.1M HEPES pH 7.5 > 3.25% PEG 8000;0.2M NaCl;0.1M NaCaco pH 5.5 > 4.20% PEG 1e500;0.2M MgCl2;0.1M Tris pH 8.5 > Second, my crystals grow within three days, and there is no phosphate in > the protein solution. > Finally, in fact, I tried crystal inoculation before, but did not find any > change in crystal shape. Maybe my operation was also wrong. I sucked out > the crystal, mashed it, diluted it in series, and removed the primary > crystal condition, and the protein concentration did not change, nor did > the concentration of the pool liquid change greatly > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
