that reminds me, for new trials you may want to try batch crystallisation under oil in Terazaki plates, or maybe microdialysis to avoid the liquid-air interface and thus skin formation
Mark van Raaij Dpto de Estructura de Macromoleculas, lab B5B Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. +34 91 585 4616 (internal 432092) > On 17 Sep 2025, at 11:00, Nukri Sanishvili > <[email protected]> wrote: > > Hi Amit, > I second every suggestion you've heard so far and would like to share a > couple more. You don't say how big are the crystals - if it's feasible at all > to mechanically separate them from the skin. If they are big, and the skin is > on a harder side, sometimes just tapping on the skin will cause the crystals > to dislodge. Ions ago, before there were array of tools available for every > occasion, I used to stretch bits of plastic over a flame to create plastic > "cat whiskers". > You may also want to try using the current crystals for seeding. With > seeding, the crystals will grow quicker, potentially before the skin forms. > Good luck! > Nukri > > On Wed, Sep 17, 2025, 5:58 AM amit gaur > <[email protected] > <mailto:[email protected]>> wrote: >> Hi everyone, >> >> I am facing a problem during crystal freezing. My crystals are surrounded by >> skin and not separating from skin during crystal mounting. It seems that >> crystals are embedded in the skin. Has anyone experienced a similar >> situation and handled this situation? >> >> Dr. Amit Gaur, >> Department of Biomedical Science, >> College of Medicine, >> Florida State University, >> 1115 W Call St, Tallahassee, FL,32304 >> <https://www.google.com/maps/search/1115+W+Call+St,+Tallahassee,+FL,32304?entry=gmail&source=g> >> >> >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
