Thanks for helpful suggestions! On Tue, Sep 16, 2025 at 6:52 PM Diana Tomchick < [email protected]> wrote:
> Sometimes when you transfer the crystals to a cryoprotectant that includes > either glycerol or ethylene glycol, it helps to solubilize the skin and > makes it easier to remove from the crystal using the techniques already > mentioned. I often wait until the very last step of increasing > cryoprotectant to the final solution before trying to remove the skin for > that reason. > > Diana > > ************************************************** > Diana R. Tomchick > Professor > Departments of Biophysics and Biochemistry > UT Southwestern Medical Center > 5323 Harry Hines Blvd. > Rm. ND10.214A > Dallas, TX 75390-8816 > [email protected] > (214) 645-6383 (phone) > (214) 645-6353 (fax) > > > > On Sep 16, 2025, at 3:57 PM, amit gaur < > [email protected]> wrote: > Hi everyone, > > I am facing a problem during crystal freezing. My crystals are surrounded > by skin and not separating from skin during crystal mounting. It seems that > crystals are embedded in the skin. Has anyone experienced a similar > situation and handled this situation? > > *Dr. Amit Gaur, * > *Department of Biomedical Science,* > *College of Medicine,* > *Florida State University,* > *1115 W Call St, Tallahassee, FL,32304* > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1__;!!MznTZTSvDXGV0Co!AxI31CI4R2fqFYarjt_b49GVlj--wh2kgUCK-Dz-rKMQVl2O0uM1RBbE1WE_MgxWXJb_vtHsVkqJMp59OOOYP7pVN3t_S-HLTw76W5TuIjREdWY$> > > > > ------------------------------ > > UT Southwestern > > Medical Center > > The future of medicine, today. > -- *Dr. Amit Gaur, * *Department of Biomedical Science,* *College of Medicine,* *Florida State University,* *1115 W Call St, Tallahassee, FL,32304* ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
