Sometimes when you transfer the crystals to a cryoprotectant that includes either glycerol or ethylene glycol, it helps to solubilize the skin and makes it easier to remove from the crystal using the techniques already mentioned. I often wait until the very last step of increasing cryoprotectant to the final solution before trying to remove the skin for that reason.
Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 [email protected] (214) 645-6383 (phone) (214) 645-6353 (fax) On Sep 16, 2025, at 3:57 PM, amit gaur <[email protected]> wrote: Hi everyone, I am facing a problem during crystal freezing. My crystals are surrounded by skin and not separating from skin during crystal mounting. It seems that crystals are embedded in the skin. Has anyone experienced a similar situation and handled this situation? Dr. Amit Gaur, Department of Biomedical Science, College of Medicine, Florida State University, 1115 W Call St, Tallahassee, FL,32304 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1__;!!MznTZTSvDXGV0Co!AxI31CI4R2fqFYarjt_b49GVlj--wh2kgUCK-Dz-rKMQVl2O0uM1RBbE1WE_MgxWXJb_vtHsVkqJMp59OOOYP7pVN3t_S-HLTw76W5TuIjREdWY$> ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
