to add to the great tips just to add that if the skin does not give much background you can leave (some of) it on. if it was a new project I'd probably mount some crystals with the skin left on (minimal manipulation) and also try some where I removed the skin. In the end it might be a trade-off with removing the skin giving less background, but increased manipulation of the crystals could also decreasing diffraction. Mark van Raaij Dpto de Estructura de Macromoleculas, lab B5B Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. +34 91 585 4616 (internal 432092)
> On 17 Sep 2025, at 00:55, amit gaur > <[email protected]> wrote: > > Thanks for helpful suggestions! > > On Tue, Sep 16, 2025 at 6:52 PM Diana Tomchick > <[email protected] > <mailto:[email protected]>> wrote: >> Sometimes when you transfer the crystals to a cryoprotectant that includes >> either glycerol or ethylene glycol, it helps to solubilize the skin and >> makes it easier to remove from the crystal using the techniques already >> mentioned. I often wait until the very last step of increasing >> cryoprotectant to the final solution before trying to remove the skin for >> that reason. >> >> Diana >> >> ************************************************** >> Diana R. Tomchick >> Professor >> Departments of Biophysics and Biochemistry >> UT Southwestern Medical Center >> 5323 Harry Hines Blvd. >> Rm. ND10.214A >> Dallas, TX 75390-8816 >> [email protected] >> (214) 645-6383 (phone) >> (214) 645-6353 (fax) >> >> >> >>> On Sep 16, 2025, at 3:57 PM, amit gaur >>> <[email protected] >>> <mailto:[email protected]>> wrote: >>> Hi everyone, >>> >>> I am facing a problem during crystal freezing. My crystals are surrounded >>> by skin and not separating from skin during crystal mounting. It seems that >>> crystals are embedded in the skin. Has anyone experienced a similar >>> situation and handled this situation? >>> >>> Dr. Amit Gaur, >>> Department of Biomedical Science, >>> College of Medicine, >>> Florida State University, >>> 1115 W Call St, Tallahassee, FL,32304 >>> >>> >>> >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>> <https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1__;!!MznTZTSvDXGV0Co!AxI31CI4R2fqFYarjt_b49GVlj--wh2kgUCK-Dz-rKMQVl2O0uM1RBbE1WE_MgxWXJb_vtHsVkqJMp59OOOYP7pVN3t_S-HLTw76W5TuIjREdWY$> >> >> UT Southwestern >> Medical Center >> The future of medicine, today. >> > > > > -- > Dr. Amit Gaur, > Department of Biomedical Science, > College of Medicine, > Florida State University, > 1115 W Call St, Tallahassee, FL,32304 > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
