Hi Amit, I second every suggestion you've heard so far and would like to share a couple more. You don't say how big are the crystals - if it's feasible at all to mechanically separate them from the skin. If they are big, and the skin is on a harder side, sometimes just tapping on the skin will cause the crystals to dislodge. Ions ago, before there were array of tools available for every occasion, I used to stretch bits of plastic over a flame to create plastic "cat whiskers". You may also want to try using the current crystals for seeding. With seeding, the crystals will grow quicker, potentially before the skin forms. Good luck! Nukri
On Wed, Sep 17, 2025, 5:58 AM amit gaur < [email protected]> wrote: > Hi everyone, > > I am facing a problem during crystal freezing. My crystals are surrounded > by skin and not separating from skin during crystal mounting. It seems that > crystals are embedded in the skin. Has anyone experienced a similar > situation and handled this situation? > > *Dr. Amit Gaur, * > *Department of Biomedical Science,* > *College of Medicine,* > *Florida State University,* > *1115 W Call St, Tallahassee, FL,32304 > <https://www.google.com/maps/search/1115+W+Call+St,+Tallahassee,+FL,32304?entry=gmail&source=g>* > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
