Hi All, There is a bit of information in the crystallization conditions that I would like to comment on. Crystals grow in a variety of conditions, including one that contains Na-Cacodylate buffer (#3 in the list). When this is the case, the cacodylate-containing conditions should be dropped from further optimization, or at least given the lowest priority. This is because cacodylate, containing arsenic, increases both the background and radiation damage during data collection, thus being undesirable. For the sake of completing the picture, when crystals grow only in the presence of cacodylate, minimizing its concentration should be one of the goals for optimization. Cheers, Nukri
On Thu, Dec 5, 2024 at 8:18 PM 白雪慧 <zb20193020...@cau.edu.cn> wrote: > Thank you very much for your reply to my question on how to optimize > microcrystal. In response to your suggestions and questions, I would like > to make the following reply: > First of all, my protein can grow crystals like this one in a variety of > situations. > For example, other conditions: > 1.15% PEG 8000;0.2M MgCl2;0.1M HEPES pH 7.5 > 2.20% PEG 1500;0.2M MgCl2;0.1M HEPES pH 7.5 > 3.25% PEG 8000;0.2M NaCl;0.1M NaCaco pH 5.5 > 4.20% PEG 1e500;0.2M MgCl2;0.1M Tris pH 8.5 > Second, my crystals grow within three days, and there is no phosphate in > the protein solution. > Finally, in fact, I tried crystal inoculation before, but did not find any > change in crystal shape. Maybe my operation was also wrong. I sucked out > the crystal, mashed it, diluted it in series, and removed the primary > crystal condition, and the protein concentration did not change, nor did > the concentration of the pool liquid change greatly > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
