Hi Careina,
One thing I'd mention in addition to the spot-on replies so far is that
cryoprotection strategies can influence diffraction. These look like pretty
spherulites though, so best of luck in optimization!

Andrew

On Wed, Nov 8, 2023, 10:03 AM careinaedgo...@yahoo.com <
000002531c126adf-dmarc-requ...@jiscmail.ac.uk> wrote:

> Hi all
> We have been trying with no success to crystalize a protein. Recently we
> got these strange shape "crystals". They are hard and flat but they do not
> diffract at all. Any ideas as to what could cause this?
> Careina
>
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