... and reveal by WB if the protein is tagged.
The trickiest part of all this will be to rinse the "xtal(s)" as extensively as possible in a protein&DNA-free solution before dissolving it for SDS-PAGE/WB or MS or whichever method you select, in order to avoid contamination by the protein-DNA complex present in the drop solution. Paradoxically, the good news is that if the xtal collapses/melts easily during the rinsing steps, it will mean that it is protein. Best wishes - Pascale ________________________________ De : CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> de la part de Jurgen Bosch <jxb...@case.edu> Envoyé : mercredi 8 novembre 2023 18:08 À : CCP4BB@JISCMAIL.AC.UK Objet : Re: [ccp4bb] What could these crystals be? You could also run them on a gel if you don’t want to shot with canons on small birds. Jürgen On Nov 8, 2023, at 11:33 AM, M T <michel...@gmail.com> wrote: Dear Careina, If you have easy access to mass spectrometry, you can try to fish/rince your « pumpkin seeds » and send them to mass to try to identify what is inside to see if it needs optimization or not. Best. Le 8 nov. 2023 à 16:03, 000002531c126adf-dmarc-requ...@jiscmail.ac.uk a écrit : Hi all We have been trying with no success to crystalize a protein. Recently we got these strange shape "crystals". They are hard and flat but they do not diffract at all. Any ideas as to what could cause this? Careina ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 <IMG-20231108-WA0007.jpg> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
