The reservior solution is 0.2 M NaCl2, 0.1 M HEPES pH 7.5, and 25% 3350 PEG
Protein buffer is 300 mM NaCl, 20 mM HEPES pH 7.5, 3mM TCEP
The drop contains 1.5ul protein-DNA complex and 1.5 ul reservior solution
On Wednesday, November 8, 2023 at 05:12:25 PM GMT+2,
stephen.c...@rc-harwell.ac.uk <stephen.c...@rc-harwell.ac.uk> wrote:
Hi Careina,
Without knowing what's in your protein buffer or crystallisation condition it's
hard to comment.
Best wishes,
Steve
Dr Stephen Carr
Research Complex at Harwell (RCaH)
Rutherford Appleton Laboratory
Harwell Oxford
Didcot
Oxon OX11 0FA
United Kingdom
Email stephen.c...@rc-harwell.ac.uk
tel 01235 567717
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of
careinaedgo...@yahoo.com <000002531c126adf-dmarc-requ...@jiscmail.ac.uk>
Sent: 08 November 2023 15:00
To: ccp4bb <ccp4bb@jiscmail.ac.uk>
Subject: [ccp4bb] What could these crystals be? Hi allWe have been trying with
no success to crystalize a protein. Recently we got these strange shape
"crystals". They are hard and flat but they do not diffract at all. Any ideas
as to what could cause this?Careina
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