Along the lines of mass spec analysis, but probably more accessible to many, is gel electrophoresis. Rinsing enough crystals and dissolving them in your protein buffer should give you a sample suitable for standard agarose and polyacrylamide gel electrophoresis protocols. I showed that crystals I had were protein-DNA complexes, as hoped, before I proceeded with attempts at experimental phasing. I had previously grown beautiful DNA-only crystals on accident (and they sure diffracted). Fortunately, it looks like you should have no trouble collecting enough crystals to make this work.
Hope this helps! On Wed, Nov 8, 2023, 11:33 AM M T <michel...@gmail.com> wrote: > Dear Careina, > > If you have easy access to mass spectrometry, you can try to fish/rince > your « pumpkin seeds » and send them to mass to try to identify what is > inside to see if it needs optimization or not. > > Best. > > Le 8 nov. 2023 à 16:03, 000002531c126adf-dmarc-requ...@jiscmail.ac.uk a > écrit : > > > Hi all > We have been trying with no success to crystalize a protein. Recently we > got these strange shape "crystals". They are hard and flat but they do not > diffract at all. Any ideas as to what could cause this? > Careina > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <IMG-20231108-WA0007.jpg> > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
