Hi Firdous,
Dry mineral oil usually works well, and it does not alter the
crystallisation drop composition. Even better if you are using sitting
drop. Just add enough mineral oil to cover the crystallisation drop, and
you even have a lot more time to pull your crystal out before the high
salt condition crystallises around the protein crystals. Eventual ligand
could also be added to the crystallization drop before pulling the
crystal through the mineral oil.
Cheers,
Magnus
Den 19/10/18 kl. 23:57, skrev Firdous Tarique:
Dear members
I have got beautiful crystal hits in SaltRx screens which are not
diffracting to a good resoultion. All of them are salt based condition
and I am not able to formulate a good cryoprotectant for these
crystals. I also think that in my case the poor resolution is due to a
poor cryoprotectant selection.
The conditions are as follows:
1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0
2>0.5M KCN 100mM Tris pH8.5
3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0
4>4M Sodium Nitrate 100mM Tris pH8.5
5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6
There are few more conditions but so far not able to see good
diffraction with using lower peg and glycerol based cryoprotectants.
Can anybody suggest me good cryos conditions for salt based
crystallization conditions or anything good for SaltRx crystallization
hits.
Thanks
Firdous
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--
Magnus Claesson, PhD
Lab manager, Drew-lab
Department of Biochemistry and Biophysics
Stockholm University
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