Condition #3 isn’t far from the saturated conditions for lithium sulfate. Perhaps slightly increasing the lithium sulfate concentration will work, or using a related lithium salt.
See Cryosalts: suppression of ice formation in macromolecular crystallography Acta Cryst D Volume 56<http://journals.iucr.org/d/services/archive.html>| Part 8<http://journals.iucr.org/d/issues/2000/08/00/index.html>| August 2000<http://journals.iucr.org/d/issues/2000/08/00/index.html>| Pages 996-1001 doi:10.1107/S0907444900007587<http://dx.doi.org/10.1107/S0907444900007587> Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Oct 19, 2018, at 4:57 PM, Firdous Tarique <kahkashantari...@gmail.com<mailto:kahkashantari...@gmail.com>> wrote: Dear members I have got beautiful crystal hits in SaltRx screens which are not diffracting to a good resoultion. All of them are salt based condition and I am not able to formulate a good cryoprotectant for these crystals. I also think that in my case the poor resolution is due to a poor cryoprotectant selection. The conditions are as follows: 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0 2>0.5M KCN 100mM Tris pH8.5 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0 4>4M Sodium Nitrate 100mM Tris pH8.5 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6 There are few more conditions but so far not able to see good diffraction with using lower peg and glycerol based cryoprotectants. Can anybody suggest me good cryos conditions for salt based crystallization conditions or anything good for SaltRx crystallization hits. Thanks Firdous ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
