Hi Firdous, I had success last week with a condition containing 1.5M MgCl2 which I’d tried cryo-ing with high salt before and got no diffraction. Adding dimethylsulphoxide to a final concentration of 10% v/v in the well solution worked well for me. DMSO was bound in the structure which can be a pest. Maybe other low mwt organics e.g. butane 2,3 diol, at around 10% might also work. Good luck!
David Dr. David Hargreaves Associate Principal Scientist _____________________________________________________________________ AstraZeneca Discovery Sciences, Structure & Biophysics Dr David Hargreaves. Office R1-09/Lab FL54 AstraZeneca Darwin Building Unit 310 Cambridge Science Park Milton Road Cambridge CB4 0WG telephone: +441223223546 mobile: 07525 742054 David.Hargreaves @astrazeneca.com<mailto:name.surn...@astrazeneca.com> Please consider the environment before printing this e-mail From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Firdous Tarique Sent: 19 October 2018 22:58 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] suggestions for cryoprotectant Dear members I have got beautiful crystal hits in SaltRx screens which are not diffracting to a good resoultion. All of them are salt based condition and I am not able to formulate a good cryoprotectant for these crystals. I also think that in my case the poor resolution is due to a poor cryoprotectant selection. The conditions are as follows: 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0 2>0.5M KCN 100mM Tris pH8.5 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0 4>4M Sodium Nitrate 100mM Tris pH8.5 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6 There are few more conditions but so far not able to see good diffraction with using lower peg and glycerol based cryoprotectants. Can anybody suggest me good cryos conditions for salt based crystallization conditions or anything good for SaltRx crystallization hits. Thanks Firdous ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://clicktime.symantec.com/a/1/TbJtumBIrBbqsKBPLB8RzbEf5A_QNLos2DtakHWXv9o=?d=wNv6P2QmMKzJRIeGSIntutsyNaCQtLMHktF40wAsjjTH2HI5rmHrASaAsvJd9cmveOm7oSDcQCWe_-NYh_gnKDEvQa9vr4Wu-ljsQ-CiMgGUJGUx0jsbOF2jWPFJQxPgTC5c3ckj_TxBwOSRS7oF3Ktf2S03YFjc4YlxA5IeFkw44maexOt1xM5NkXgtwx-2M2eXZX4vgGIBLf7VND_mZ1AlxnhijOZ_HzauItrCZoQfMRw5XZrjW1XAa2gqm8lQuHRXcoCqQsJRKt0U9zZ8RGtyTJ-JLLLLT5ssezzeJXpBhZg3TtXc8SzxwLdf3z2hFXlUk6kk0CHNzHq_1BHsZ6xvjf40Ny5wX-Nu6PdByrujRz0ZFVSQmMPO4X1kiPZTSd4h_u2v64LDIt5oQN5K0NtxXkmi0Skwcg%3D%3D&u=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1> ________________________________ AstraZeneca UK Limited is a company incorporated in England and Wales with registered number:03674842 and its registered office at 1 Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0AA. This e-mail and its attachments are intended for the above named recipient only and may contain confidential and privileged information. If they have come to you in error, you must not copy or show them to anyone; instead, please reply to this e-mail, highlighting the error to the sender and then immediately delete the message. For information about how AstraZeneca UK Limited and its affiliates may process information, personal data and monitor communications, please see our privacy notice at www.astrazeneca.com<https://www.astrazeneca.com> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
