Hi Firdous,
For salt conditions try malonate:
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2356-8. Epub 2003 Nov
27.
Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown
macromolecular crystals.
Holyoak T1, Fenn TD, Wilson MA, Moulin AG, Ringe D, Petsko GA.
Best,
Wally
--
Walter R.P. Novak
Associate Professor and Chair of Chemistry
Wabash College
301 W. Wabash Ave.
Crawfordsville, IN 47933
Phone: 765-361-6407
Fax: 765-361-6149
Date: Tue, 23 Oct 2018 13:29:10 -0700
From: Kevin Jin <kevin...@gmail.com>
Subject: Re: suggestions for cryoprotectant
Dear Firdous,
Probably other people already suggested above, and I don't know which kind
of protein in your case. Here is my suggestion, you may try sugar solution
or polysaccharide solution as cryoprotectant. The whole idea is to provide
alternative H-bond network to support protein freezing. Indeed, some
startups are using similar ideas to develop cryoprotectants for medical
applications.
Best,
Kevin
On Fri, Oct 19, 2018 at 2:58 PM Firdous Tarique <kahkashantari...@gmail.com>
wrote:
> Dear members
>
> I have got beautiful crystal hits in SaltRx screens which are not
> diffracting to a good resoultion. All of them are salt based condition and
> I am not able to formulate a good cryoprotectant for these crystals. I
also
> think that in my case the poor resolution is due to a poor cryoprotectant
> selection.
>
> The conditions are as follows:
>
> 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0
> 2>0.5M KCN 100mM Tris pH8.5
> 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0
> 4>4M Sodium Nitrate 100mM Tris pH8.5
> 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6
>
> There are few more conditions but so far not able to see good diffraction
> with using lower peg and glycerol based cryoprotectants.
>
> Can anybody suggest me good cryos conditions for salt based
> crystallization conditions or anything good for SaltRx crystallization
hits.
>
> Thanks
>
> Firdous
>
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--
Kevin Jin
Sharing knowledge each other is always very joyful......
Website: http://www.jinkai.org/
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