Dear Fridous Have a look at https://www.ncbi.nlm.nih.gov/pubmed/14646118 Malonate as a cryoprotectant
Or https://www.ncbi.nlm.nih.gov/pubmed/20606259 Regards Christine On Fri, Oct 19, 2018 at 2:57 PM Firdous Tarique <kahkashantari...@gmail.com> wrote: > Dear members > > I have got beautiful crystal hits in SaltRx screens which are not > diffracting to a good resoultion. All of them are salt based condition and > I am not able to formulate a good cryoprotectant for these crystals. I also > think that in my case the poor resolution is due to a poor cryoprotectant > selection. > > The conditions are as follows: > > 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0 > 2>0.5M KCN 100mM Tris pH8.5 > 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0 > 4>4M Sodium Nitrate 100mM Tris pH8.5 > 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6 > > There are few more conditions but so far not able to see good diffraction > with using lower peg and glycerol based cryoprotectants. > > Can anybody suggest me good cryos conditions for salt based > crystallization conditions or anything good for SaltRx crystallization hits. > > Thanks > > Firdous > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
