Hi Firdous, I noticed that conditions 2,4 and 5 are overall harsh agents (chaotropic agents such as KSCN (probably not KCN) and Nitrate ions ), in my own personal experience I have never been very lucky with crystals grown in nitrate ions. Condition 1 however looks a bit more soft with 'gentle' ions. May I suggest trying a sodium malonate screen or seeding in such a crystallization agent and maybe trying to cryprotect those acetate crystals in malonate which is a cryo-friendly so-called 'magic salt'. crystals grown in carboxylates such as citrate and malonate are usually easier to cryo-protect.
Hope this helps Pascal Egea On Fri, Oct 19, 2018 at 2:57 PM Firdous Tarique <kahkashantari...@gmail.com> wrote: > Dear members > > I have got beautiful crystal hits in SaltRx screens which are not > diffracting to a good resoultion. All of them are salt based condition and > I am not able to formulate a good cryoprotectant for these crystals. I also > think that in my case the poor resolution is due to a poor cryoprotectant > selection. > > The conditions are as follows: > > 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0 > 2>0.5M KCN 100mM Tris pH8.5 > 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0 > 4>4M Sodium Nitrate 100mM Tris pH8.5 > 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6 > > There are few more conditions but so far not able to see good diffraction > with using lower peg and glycerol based cryoprotectants. > > Can anybody suggest me good cryos conditions for salt based > crystallization conditions or anything good for SaltRx crystallization hits. > > Thanks > > Firdous > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry Boyer Hall room 356 611 Charles E Young Drive East Los Angeles CA 90095 office (310)-983-3515 lab (310)-983-3516 email pegea at mednet.ucla.edu ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
