Vandana,
Roger gives some good advice and I agree with Fred about not
knowing the number of mols in the AU. I routinely see solvent content in
the 75% range for many different crystals, although they tend to
diffract rather poorly. In addition, you may have to convert your search
model to poly-alanine and you may want to decrease the resolution during
MR.
Jon
--
Jonathan P. Schuermann, Ph. D.
Beamline Scientist
NE-CAT, Building 436E
Advanced Photon Source (APS)
Argonne National Laboratory
9700 South Cass Avenue
Argonne, IL 60439
email: schue...@anl.gov
Tel: (630) 252-0682
Fax: (630) 252-0687
Vandana Kukshal wrote:
hello sir ,
recently i have collected one data of 3.0 A of a protein having no
sequence homology with any known PDB .
but
while fold prediction i got 100 % identical fold with some of the
protein .
space group of my protein is P622 and showing 6 molecule in a
assymetric unit.
the homologous fold proteins are trimer protein.
can i run MR in this case . how i should proceed.
i m trying for MIR also .
regards
vandana
