Dear Changyi I do not know details about your structure, but considering you have the correct library for your ligand and your resolution is not bad, I would check a possible double conformation. Good luck Cristy
On Wed, 4 Aug 2010 16:15:55 -0400, Changyi Xue wrote > Dear all, > > In my structure, there is a ligand, whichcontains a sugar ring. In the > process of refinement, refmac alwaystried to distort the sugar ring to fit > into the density. Is there anyway to fix or restrain the ring conformation > more tightly? I know CNShas such function, just wandering if refmac could do > it also. > > all suggestions are welcome. > > changyi ------------------------------------------------------------- Profa Dra Maria Cristina Nonato Laboratório de Cristalografia de Proteínas Departamento de Física e Química Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP Fone: +55.16.3602.4432 Fax: +55.16.3602.4880 [email protected]
