Hi, well are you saying you are getting microcrystals with large
(infite?) dilution of the precipitant or complex? at some point there
must be change. try seeding? or then you are getting salt crystals for
some reason? do you have a control drop?
best of luck,
tommi
On Oct 11, 2010, at 8:34 AM, Dilip Badgujar wrote:
Thanks guys for your reply.
This to clear the some doubt about my querry.
1.When I lowered the complex conc. then there are too many
microcrystals.Same thing happen when I lower the precipitant conc.
2.My proteins pI is 6.45 and I am using a 50mM Tris pH 7.5 and 300mM
NaCl
3.Before making complex I spun the protein @14,000rpm 10min.
the complex solution is clear.
Regards
Dilip
Tommi Kajander, Ph.D.
Structural Biology and Biophysics
Institute of Biotechnology
University of Helsinki
Viikinkaari 1
(P.O. Box 65)
00014 Helsinki
Finland
p. +358-9-19158903
tommi.kajan...@helsinki.fi
