Dear all --
I have a very "stupid" question/remark concerning this protein
concentration matter. Let say one has two different proteins, of 7 and
700 kDa, respectively. I guess that 20 mg/mL concentration would be
considered as a high concentration for the first protein, and probably
"less high" for the second. In order to be able to compare protocols,
is the notation mg/mL of proper choice? Would mM be a better option?
Or do we all assume working on the same averaged size sample?
Kind regards.
-- Leo --
made iPhone... Sorry for the eventual mistakes !
On 2010/03/06, at 2:44, "Murray, Michael (NIH/NIEHS)" <murra...@niehs.nih.gov
> wrote:
Jan,
There is some good advice on dealing with proteins that do not
self-aggregate here. Assuming that most or all of your drops are
clear this
might be useful.
<http://web.emeraldbiosystems.com/blog/bid/24287/All-drops-clear-Back-to-Old
-School-protein-crystallization>
Good luck,
Mike
Michael S. Murray, Ph.D.
National Institute of Environmental Health Sciences
MD E3-01
P.O. Box 12233
Research Triangle Park, NC 27709
Phone: (919) 541-0268
On 3/5/10 9:02 AM, "Jan Rash" <jan...@googlemail.com> wrote:
Dear All,
This is about the crystallization of the macromolecular complex
which is
highly soluble and shows no signs of the aggregation (even at high
concentration). We have tried several salts, precipitants and even
high
protein concentration (around 20g/L) for its crystallization
without any
genuine hit. Any suggestions for growing the crystals of this
macromolecular
complex will be highly appreciated.
Thanks,
Jan
