Whilst I agree with Leo that mM is a much more satisfying or rigorous unit of concentration, the huge variability of solubility observed in proteins, and even between point-mutants means we may as well use gigatonnes / firkin, for all the difference it makes wrt comparing crystallisation trials.
IMHO, every construct is different, and if 20 mg/ml gives clear drops, double it and try again. Hth, Dave -- Delivered via an Android. On Mar 6, 2010 9:24 AM, <b...@freesurf.fr> wrote: Dear Leo, here's a little gedankenexperiment: imagine cross-linking those solutions you have, say with glutaraldehyde. Would you say this will *improve* solubility because there are now less molecules? Best wishes, Sebastiaan Werten. > Dear all -- > > I have a very "stupid" question/remark concerning this protein > concentration m...
