In addition to what Hermann said (20g/l is what I was taught to be the average starting concentration for crystallisation experiment, so it's not particularly high) I would like to add that, provided you have enough material, you can estimate the solubility of your protein by concentrating it in a concentrator and plotting the concentration over time without resuspending the supernatant. That's only a rough estimate but it's a starting point.
On Fri, Mar 05, 2010 at 03:02:55PM +0100, Jan Rash wrote: > Dear All, > > This is about the crystallization of the macromolecular complex which is > highly soluble and shows no signs of the aggregation (even at high > concentration). We have tried several salts, precipitants and even high > protein concentration (around 20g/L) for its crystallization without any > genuine hit. Any suggestions for growing the crystals of this macromolecular > complex will be highly appreciated. > > Thanks, > > Jan -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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