Dear Jan,
20g/L is not a high protein concentration. High is 80-100g/L and that is
what I would try. Remember, a protein crystal contains ~500g/L protein.
If you do not observe any precipitate, I would go to very high
precipitant concentrations. I once found protein crystals in between
ammonium sulfate crystals in some almost dried-out crystallization
experiments. These crystals diffracted X-rays reasonably well and we
were able to solve the structure.
Good luck,
Herman
________________________________
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On
Behalf Of Jan Rash
Sent: Friday, March 05, 2010 3:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystallization of a macromolecular complex
Dear All,
This is about the crystallization of the macromolecular complex
which is highly soluble and shows no signs of the aggregation (even at
high concentration). We have tried several salts, precipitants and even
high protein concentration (around 20g/L) for its crystallization
without any genuine hit. Any suggestions for growing the crystals of
this macromolecular complex will be highly appreciated.
Thanks,
Jan
