Jan, There is some good advice on dealing with proteins that do not self-aggregate here. Assuming that most or all of your drops are clear this might be useful.
<http://web.emeraldbiosystems.com/blog/bid/24287/All-drops-clear-Back-to-Old -School-protein-crystallization> Good luck, Mike Michael S. Murray, Ph.D. National Institute of Environmental Health Sciences MD E3-01 P.O. Box 12233 Research Triangle Park, NC 27709 Phone: (919) 541-0268 On 3/5/10 9:02 AM, "Jan Rash" <jan...@googlemail.com> wrote: > Dear All, > > This is about the crystallization of the macromolecular complex which is > highly soluble and shows no signs of the aggregation (even at high > concentration). We have tried several salts, precipitants and even high > protein concentration (around 20g/L) for its crystallization without any > genuine hit. Any suggestions for growing the crystals of this macromolecular > complex will be highly appreciated. > > Thanks, > > Jan
